COMPOSITION ANALYSIS BY GC-MS OF THREE SAMPLES

通过 GC-MS 对三个样品进行成分分析

• 批准号: 8361856
• 负责人: Parastoo Azadi
• 金额: $ 0.18万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-02-01 至 2012-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361856
• 关键词: Acetylation; Blood capillaries; Funding; Gases; Glycosides; Grant; Guanine + Cytosine Composition; Inositol; Methanol; Methods; National Center for Research Resources; Principal Investigator; Reagent; Research; Research Infrastructure; Resources; Sampling; Series; Silicon Dioxide; Source; Time; Tube; United States National Institutes of Health; acetic anhydride; capillary; cost; pyridine

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Methods: Glycosyl composition by GC-MS The samples (~20 ¿g each) were placed in screw-cap tubes, 2 ¿L 1 mg/mL inositol was added as internal standard, and the samples were lyophilized. Methyl glycosides then were prepared from the dried samples by methanolysis with 3 M HCl in methanol at 100 ¿C for 2 h followed by re-N-acetylation with pyridine and acetic anhydride in methanol. The preceding methanolysis and re-N-acetylation steps were repeated two times. The samples then were per-O-trimethylsilylated (TMS) with a Tri-Sil reagent (Thermo Scientific) at 80 ¿C for 0.5 h. Analysis of the TMS methyl glycosides was performed on a Hewlett Packard Series II 5890 gas chromatograph equipped with a Supelco EC-1 fused silica capillary column (30m ¿ 0.25 mm ID) and interfaced to a Hewlett Packard 5970 MSD.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供 该子项目的主要支持。 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源的子项目可能列出的总成本。 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 方法： GC-MS 测定的糖基组成 将样品（每个约 20 克）放入螺旋盖管中，2 克添加 L 1 mg/mL 肌醇作为内标，然后将样品冻干，然后用 3 M HCl 的甲醇溶液在 100 °C 下进行甲醇分解来制备甲基糖苷。 C 2小时，然后用吡啶和乙酸酐在甲醇中进行再N-乙酰化，然后将样品用Tri-O-三甲基甲硅烷基化(TMS)重复两次。 Sil 试剂（Thermo Scientific）在 80¿ C 0.5 小时，在配有 Supelco EC-1 熔融石英毛细管柱（内径 30m × 0.25 mm）并与 Hewlett Packard 5970 MSD 连接的 Hewlett Packard 系列 II 5890 气相色谱仪上进行 TMS 甲基糖苷分析。