Orbitrap Fusion Tribrid Mass Spectrometer

Orbitrap 融合三联体质谱仪

• 批准号: 8734751
• 负责人: Parastoo Azadi
• 金额: $ 75万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-15 至 2015-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8734751
• 关键词: Anthrax disease; Atherosclerosis; Carbohydrates; Cardiac; Communities; Complex; Country; Disease; Educational workshop; Embolism; Funding; Glycosaminoglycans; HIV vaccine; Heart Diseases; Institutes; Laboratories; Lafora Disease; Liquid Chromatography; Lung diseases; Mass Spectrum Analysis; Muscular Dystrophies; Pulmonary Fibrosis; Research; Research Personnel; Research Project Grants; Resolution; Services; System; Training; United States; United States National Institutes of Health; Vaccine Research; Venous; Xenograft procedure; flexibility; instrument; instrumentation; mass spectrometer; nano

DESCRIPTION (provided by applicant): The Analytical Service and Training Laboratory (ASTL) at the Complex Carbohydrate Research Center is requesting funds to purchase a Thermo Scientific Orbitrap Tribrid Fusion mass spectrometer and nano-liquid chromatography system that will be dedicated to glycomics, glycoproteomics and glycosaminoglycan research service projects from a large number of NIH-Investigators from institutes all across the United States. The Orbitrap Fusion at ASTL will have a major impact on many disease related biomedical glycomics projects. This research is significant for muscular dystrophy, HIV vaccine research, heart and lung disease, atherosclerosis and venous thrombo-embolism, cardiac xenotransplantation, Lafora disease, pulmonary fibrosis, anthrax, and many others. The Orbitrap Fusion has unique features such as high sensitivity and resolution, HCD/ETD fragmentation capability, and unprecedented flexibility, which are necessary for all our glycomics and glycoproteomics proposed research projects. Existing LC-MS instrumentation in the facility includes an older LTQ-Orbitrap Discovery, which is not upgradable to ETD/HCD capability and has been discontinued. The number of research groups that will be impacted by the acquisition of the proposed Orbitrap Fusion instrument at the ASTL spans the entire country. Just in the past five years, more than 60 NIH-funded investigators from all over U.S have accessed ASTL's instrumentation and its unique expertise to advance their own carbohydrate research projects. The Orbitrap Fusion will also be utilized in several annual hands-on workshops to train more glycoscientists, and to bring cutting edge mass spectrometry expertise to the larger glycoscience community.

描述（由申请人提供）：复杂碳水化合物研究中心的分析服务和培训实验室 (ASTL) 正在申请资金购买 Thermo Scientific Orbitrap Tribrid Fusion 质谱仪和纳米液相色谱系统，该系统将专用于糖组学、糖蛋白质组学和来自美国各地研究所的大量 NIH 研究人员的糖胺聚糖研究服务项目。 ASTL 的 Orbitrap Fusion 将对许多疾病相关的生物医学糖组学项目产生重大影响。这项研究对于肌营养不良、艾滋病毒疫苗研究、心肺疾病、动脉粥样硬化和静脉血栓栓塞、心脏异种移植、拉福拉病、肺纤维化、炭疽病等具有重要意义。 Orbitrap Fusion 具有独特的功能，如高灵敏度和分辨率、HCD/ETD 裂解能力和前所未有的灵活性，这些都是我们所有糖组学和糖蛋白质组学拟议研究项目所必需的。该设施中现有的 LC-MS 仪器包括较旧的 LTQ-Orbitrap Discovery，该仪器无法升级至 ETD/HCD 功能，并且已停产。 ASTL 购买拟议的 Orbitrap Fusion 仪器将影响全国范围内的研究小组数量。仅仅在过去的五年里，来自美国各地的 60 多名 NIH 资助的研究人员就使用了 ASTL 的仪器及其独特的专业知识来推进他们自己的碳水化合物研究项目。 Orbitrap Fusion 还将在多个年度实践研讨会中使用，以培训更多的糖科学家，并将尖端的质谱专业知识带给更大的糖科学界。

项目成果

Highly active CAR T cells that bind to a juxtamembrane region of mesothelin and are not blocked by shed mesothelin.

高活性 CAR T 细胞与间皮素的近膜区域结合，并且不会被脱落的间皮素阻断。

• 发表时间: 2022-05-10
• 影响因子: 11.1
• 作者: Liu, Xiufen;Onda, Masanori;Watson, Nathan;Hassan, Raffit;Ho, Mitchell;Bera, Tapan K;Wei, Junxia;Chakraborty, Anirban;Beers, Richard;Zhou, Qi;Shajahan, Asif;Azadi, Parastoo;Zhan, Jingyu;Xia, Di;Pastan, Ira
• 通讯作者: Pastan, Ira

Distinct roles of N- and O-glycans in cellulase activity and stability.

N-和O-聚糖在纤维素酶活性和稳定性中的不同作用。

• 发表时间: 2017-12-26
• 影响因子: 11.1
• 作者: Amore, Antonella;Knott, Brandon C;Supekar, Nitin T;Shajahan, Asif;Azadi, Parastoo;Zhao, Peng;Wells, Lance;Linger, Jeffrey G;Hobdey, Sarah E;Vander Wall, Todd A;Shollenberger, Todd;Yarbrough, John M;Tan, Zhongping;Crowley, Michael F;Himmel, M
• 通讯作者: Himmel, M

Deoxyinosine and 7-Deaza-2-Deoxyguanosine as Carriers of Genetic Information in the DNA of Campylobacter Viruses.

脱氧肌苷和 7-Deaza-2-脱氧鸟苷作为弯曲杆菌病毒 DNA 中遗传信息的载体。

• 发表时间: 2019-12-01
• 作者: Crippen, Clay S;Lee, Yan;Hutinet, Geoffrey;Shajahan, Asif;Sacher, Jessica C;Azadi, Parastoo;de Crécy;Weigele, Peter R;Szymanski, Christine M
• 通讯作者: Szymanski, Christine M

Generation of 13C-Labeled MUC5AC Mucin Oligosaccharides for Stable Isotope Probing of Host-Associated Microbial Communities.

生成 13C 标记的 MUC5AC 粘蛋白寡糖，用于宿主相关微生物群落的稳定同位素探测。

• DOI: 10.1021/acsinfecdis.8b00296
• 发表时间: 2019-01-09
• 期刊: ACS infectious diseases
• 影响因子: 5.3
• 作者: Clayton Evert;Tina Loesekann;G. Bhat;A. Shajahan;R. Sonon;P. Azadi;R. Hunter
• 通讯作者: R. Hunter

N-Linked Glycosylation on Anthrax Toxin Receptor 1 Is Essential for Seneca Valley Virus Infection.

炭疽毒素受体 1 上的 N 联糖基化对于塞内卡谷病毒感染至关重要。

• 发表时间: 2021
• 作者: Jayawardena, Nadishka;Miles, Linde A;Burga, Laura N;Rudin, Charles;Wolf, Matthias;Poirier, John T;Bostina, Mihnea
• 通讯作者: Bostina, Mihnea