Neurogenetic Approaches to Study Directed Behavior in Drosophila

研究果蝇定向行为的神经遗传学方法

• 批准号: 8671258
• 负责人: Marco Gallio
• 金额: $ 32.58万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-01 至 2019-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8671258
• 关键词: Address; Affect; Animals; Behavior; Behavior Control; Behavioral; Brain; Calcium; Cells; Chromosome Mapping; Complex; Detection; Diffusion; Diphtheria Toxin; Disease; Dissection; Drosophila genus; Environment; Funding; Genetic; Genetic Techniques; Goals; Hereditary Disease; Image; Imaging technology; Instinct; Label; Life; Logic; Maps; Mediating; Microscopy; Modality; Modeling; Molecular Genetics; Monitor; Motor output; Nervous System Physiology; Nervous system structure; Neural Pathways; Neurons; Olfactory Pathways; Output; Pathway interactions; Pattern; Population; Positioning Attribute; Process; Property; Sensory; Site; Stimulus; Synapses; System; Temperature; Tetanus Toxin; Toxin; Transgenes; Transgenic Organisms; Work; base; candidate identification; cell type; fly; insight; multi-photon; neural circuit; neurogenetics; preference; programs; public health relevance; receptor; reconstitution; red fluorescent protein; relating to nervous system; response; sensory stimulus; tool

DESCRIPTION (provided by applicant): Our long term goal is to provide a framework to understand how a simple, innate behavior emerges from the activity of the nervous system. For this, we will apply a battery of molecular genetics techniques and imaging technologies to study the circuit mechanisms underlying temperature preference in Drosophila -a system ideally suited for a comprehensive genetic and molecular dissection of neural circuits and behaviors. We have recently shown that temperature is represented by a map of activity created by sensory afferents at the first relay station in the fly brain. We now propose to study how this map is processed by central neural circuits and transformed into directed behavior. To accomplish our overall objectives, we will use a combination of targeted photo-activation of GFP, GRASP, ChR2-mediated circuit mapping and genetic expression of effector molecules to: 1) Identify ascending neuronal populations receiving temperature information and representing it to higher brain centers. 2) Study their tuning and properties, and narrow our search to the key pathways that mediate temperature navigation and preference. 3) Determine their connectivity to higher brain centers, study the transformation of stimuli at each station in the circuit and eventually track the descending pathways that control behavior. This work is expected to contribute to our general understanding of the wiring logic of the neural circuits that control innate behavior in animals, and potentially provide insights on how genetic conditions which affect wiring can result in compromised circuit function and altered behavior.

描述（由申请人提供）：我们的长期目标是提供一个框架来理解简单的、先天的行为是如何从神经系统的活动中产生的。为此，我们将应用一系列分子遗传学技术和成像技术来研究果蝇温度偏好背后的回路机制——这是一个非常适合对神经回路和行为进行全面遗传和分子解剖的系统。我们最近表明，温度是由果蝇大脑中第一个中继站的感觉传入产生的活动图来表示的。我们现在建议研究这张地图如何 由中枢神经回路处理并转化为定向行为。为了实现我们的总体目标，我们将结合使用 GFP、GRASP、ChR2 介导的电路映射和效应分子基因表达的靶向光激活来：1) 识别接收温度信息并将其表示给更高大脑中心的上行神经元群。 2）研究它们的调节和特性，并将我们的搜索范围缩小到介导温度导航和偏好的关键途径。 3）确定它们与高级大脑中心的连接，研究回路中每个站点的刺激转换，并最终跟踪控制行为的下行路径。这项工作预计将有助于我们对控制动物先天行为的神经回路接线逻辑的总体理解，并可能提供关于影响接线的遗传条件如何导致回路功能受损和行为改变的见解。