Adapting Neurogenetic Technologies for Use in the Desert-dwelling Fly Drosophila Mojavensis

将神经遗传学技术应用于沙漠果蝇莫哈文西斯

基本信息

批准号：
10575291
负责人：
Marco Gallio
金额：
$ 42万
依托单位：
NORTHWESTERN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-01-01 至 2024-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10575291
关键词：
Affect Animal Behavior Animal Model Animals Behavior Behavioral Assay Biological Assay Biological Models Brain CRISPR/Cas technology Caenorhabditis elegans Cells Color Communities Comparative Study Decision Making Disease Drosophila genus Drosophila melanogaster Dryness Environment Evolution Eye Gene Transfer Techniques Genes Genetic Goals Habitats Homologous Gene Human Humidity Image Individual Label Laboratories Maps Mediating Mediator Methods Mexico Modeling Molecular Mus Mutate Nematoda Nervous System Nervous System Physiology Neurobiology Neurons Neurosciences Research Pain Phenotype Physiology Process Protocols documentation Rattus Reagent Receptor Gene Resources Sclera Sensory Shapes Signal Transduction Site Southwestern United States Standardization System Techniques Technology Temperature Testing Transgenes Transgenic Organisms Work Zebrafish calcium indicator cell type comparative design expectation extreme temperature flexibility fly gene conservation genome editing mutant neurogenetics optogenetics preference promoter receptor response sensory stimulus sensory system site-specific integration somatosensory

项目摘要

PROJECT SUMMARY: Our ultimate goal is to elucidate how sensory processing occurs in the brain at the level of individual cells and circuits, and how this leads to directed behavior through the process of decision making. Here, we will create neurogenetic reagents that will help introduce the desert fly Drosophila mojavensis as a new comparative model system to study the neurobiology of temperature and humidity sensing and preference. We will use CRISPR/Cas9 genome editing technology and site-specific integration to produce D. mojavensis strains that can be flexibly used to silence, image the activity of, or activate select cell types that process temperature and humidity signals at the periphery and in the brain. Our expectation is that, once the key foundational work has been completed, our reagents will help us and others to design powerful comparative studies to establish conservation and divergence between this extremophile species and its laboratory relative D. melanogaster, and ultimately help identify general principles used in sensory processing by all animals.

项目概要：我们的最终目标是阐明大脑中单个细胞水平的感觉处理是如何发生的电路，以及它如何通过决策过程导致定向行为。在这里，我们将创建神经遗传学试剂将有助于引入沙漠蝇果蝇莫哈文西斯作为新的比较研究温度和湿度感知和偏好的神经生物学的模型系统。我们将使用 CRISPR/Cas9 基因组编辑技术和位点特异性整合可产生 D. mojavensis 菌株，可灵活用于沉默、成像或激活处理温度和温度的选定细胞类型周围和大脑中的湿度信号。我们的期望是，一旦关键的基础工作完成完成后，我们的试剂将帮助我们和其他人设计强大的比较研究来建立这种极端微生物物种与其实验室亲戚黑腹果蝇之间的保护和分歧，并最终帮助确定所有动物感官处理中使用的一般原则。