Peri-Conceptional Biomonitoring

围孕期生物监测

• 批准号: 8668059
• 负责人: Christina Porucznik
• 金额: $ 33.51万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-15 至 2016-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8668059
• 关键词: Address; Affect; Animal Model; Biological; Biological Markers; Biological Monitoring; Blood; Body Temperature; Cervical; Chemical Exposure; Chemicals; Collection; Communities; Conceptions; Couples; Data; Development; Disinfection; Dose; Educational process of instructing; Environmental Exposure; Environmental Monitoring; Environmental Pollution; Epidemiologic Studies; Epidemiology; Exposure to; Female; Fertile Period; Fertility; Future; Half-Life; Hormonal; Hormones; Human; Human Development; Individual; Liquid substance; Measurement; Measures; Methods; Monitor; Outcome; Ovulation; Participant; Patient Self-Report; Phase; Phenols; Pilot Projects; Population; Pregnancy; Prenatal care; Principal Investigator; Probability; Questionnaires; Recruitment Activity; Reporting; Sampling; Seminal fluid; Specimen; Staging; System; Time; Urine; Visit; bisphenol A; career; cohort; cost effective; design; drinking water; implantation; in vivo; kinetosome; male; novel; novel strategies; ovulation time; population based; pregnant; prenatal; prospective; repository; reproductive; time interval

DESCRIPTION (provided by applicant): This proposal will address 1) accurate assessment of environmental exposures during the earliest phases of human development, and 2) association of these exposures with semen quality and time to pregnancy in a community-dwelling cohort of 300 couples trying to conceive. The contaminants selected for measurement, disinfection by-products (DBP) and bisphenol-A (BPA), have been associated with adverse reproductive outcomes in animal models and in limited epidemiologic studies. Exposure to these and other environmental contaminants is not well measured via traditional epidemiologic means such as participant recall using questionnaires or environmental monitoring in early pregnancy, such as at the time of the first prenatal visit, or ecologic measures such as assignment of contaminant levels reported from drinking water system monitoring. Biomarkers can allow for more precise estimates of internal dose, but for transient exposures and chemicals with short half-lives, the biological specimen must be collected at the right time in order to properly classify individual exposure in relation to critical developmental windows. Biomarkers collected in early pregnancy may be too late to detect peri-conceptional exposure if the exposure was transient or if the relevant chemical had a short half-life. Peri-ovulational exposures that affect probability of conception would also be missed in this type of design, as couples who fail to conceive will not enter into prenatal care. SPECIFIC AIMS 1. Conduct prospective, peri-ovulational biomonitoring for disinfection by-products (DBP) and bis-phenol-A (BPA) in a human preconception cohort using a novel strategy of self-collection of environmental and biological specimens triggered by observation of fertility signs in a community-dwelling population. 2. Evaluate the association between prospectively measured exposures in the male and the female and the reproductive outcomes of semen quality and time to pregnancy. 3. Quantify the degree of misclassification bias generated by retrospective exposure assessment methods in common use (participant self-report of past exposure using a recall questionnaire and/or archival environmental monitoring data) compared to prospective peri-ovulational biomonitoring and sampling. The proposal is to recruit a pre-conception cohort, conduct prospective exposure assessment via biomarkers and questionnaires, evaluate the association of exposure with time to pregnancy, and compare prospectively measured exposures with retrospectively estimated exposures. Collected biospecimens will be banked as a repository for future analyses. Creation of this cohort and specimen bank will launch the independent career of Principal Investigator (Early Stage) Porucznik.

描述（由申请人提供）：该提案将解决 1) 对人类发育最早阶段的环境暴露的准确评估，以及 2) 在社区居住的 300 对夫妇中研究这些暴露与精液质量和怀孕时间的关系怀孕。在动物模型和有限的流行病学研究中，选择用于测量的污染物、消毒副产物 (DBP) 和双酚 A (BPA) 与不良生殖结果相关。通过传统的流行病学手段，例如使用问卷调查的参与者回忆或怀孕早期的环境监测（例如在第一次产前检查时）或生态措施（例如分配报告中的污染物水平），无法很好地测量这些和其他环境污染物的暴露情况。饮用水系统监测。生物标志物可以更精确地估计内部剂量，但对于瞬时暴露和半衰期短的化学品，必须在正确的时间收集生物样本，以便根据关键发育窗口对个体暴露进行正确分类。如果暴露是短暂的或相关化学物质的半衰期较短，则在怀孕早期收集的生物标志物可能来不及检测围孕期暴露。在这种类型的设计中，影响受孕概率的围排卵暴露也会被忽略，因为未能受孕的夫妇不会进入产前护理。具体目标 1. 使用一种自行收集环境和生物样本的新策略，对人类孕前队列中的消毒副产物 (DBP) 和双酚 A (BPA) 进行前瞻性围排卵生物监测。社区居住人口的生育迹象。 2. 评估男性和女性前瞻性测量的暴露与精液质量和怀孕时间的生殖结果之间的关联。 3. 与前瞻性围排卵生物监测和采样相比，量化常用回顾性暴露评估方法（参与者使用回忆问卷和/或档案环境监测数据自我报告过去暴露情况）产生的错误分类偏差的程度。该提案是招募一个孕前队列，通过生物标志物和问卷进行前瞻性暴露评估，评估暴露与怀孕时间的关联，并将前瞻性测量的暴露与回顾性估计的暴露进行比较。收集到的生物样本将被存入银行，作为未来分析的存储库。该队列和样本库的创建将开启首席研究员（早期阶段）Porucznik 的独立职业生涯。