Peri-Conceptional Biomonitoring

围孕期生物监测

• 批准号: 8848379
• 负责人: Christina Porucznik
• 金额: $ 32.99万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-15 至 2017-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8848379
• 关键词: Address; Affect; Animal Model; Biological; Biological Markers; Biological Monitoring; Blood; Body Temperature; Cervical; Chemical Exposure; Chemicals; Collection; Communities; Conceptions; Couples; Data; Development; Disinfection; Dose; Educational process of instructing; Environmental Exposure; Environmental Monitoring; Environmental Pollution; Epidemiologic Studies; Epidemiology; Exposure to; Female; Fertile Period; Fertility; Future; Half-Life; Hormonal; Hormones; Human; Human Development; Individual; Liquid substance; Measurement; Measures; Methods; Monitor; Outcome; Ovulation; Participant; Patient Self-Report; Phase; Phenols; Pilot Projects; Population; Pregnancy; Prenatal care; Principal Investigator; Probability; Questionnaires; Recruitment Activity; Reporting; Sampling; Seminal fluid; Specimen; Staging; System; Time; Urine; Visit; bisphenol A; career; cohort; cost effective; design; drinking water; implantation; in vivo; kinetosome; male; novel; novel strategies; ovulation time; population based; pregnant; prenatal; prospective; repository; reproductive; time interval

DESCRIPTION (provided by applicant): This proposal will address 1) accurate assessment of environmental exposures during the earliest phases of human development, and 2) association of these exposures with semen quality and time to pregnancy in a community-dwelling cohort of 300 couples trying to conceive. The contaminants selected for measurement, disinfection by-products (DBP) and bisphenol-A (BPA), have been associated with adverse reproductive outcomes in animal models and in limited epidemiologic studies. Exposure to these and other environmental contaminants is not well measured via traditional epidemiologic means such as participant recall using questionnaires or environmental monitoring in early pregnancy, such as at the time of the first prenatal visit, or ecologic measures such as assignment of contaminant levels reported from drinking water system monitoring. Biomarkers can allow for more precise estimates of internal dose, but for transient exposures and chemicals with short half-lives, the biological specimen must be collected at the right time in order to properly classify individual exposure in relation to critical developmental windows. Biomarkers collected in early pregnancy may be too late to detect peri-conceptional exposure if the exposure was transient or if the relevant chemical had a short half-life. Peri-ovulational exposures that affect probability of conception would also be missed in this type of design, as couples who fail to conceive will not enter into prenatal care. SPECIFIC AIMS 1. Conduct prospective, peri-ovulational biomonitoring for disinfection by-products (DBP) and bis-phenol-A (BPA) in a human preconception cohort using a novel strategy of self-collection of environmental and biological specimens triggered by observation of fertility signs in a community-dwelling population. 2. Evaluate the association between prospectively measured exposures in the male and the female and the reproductive outcomes of semen quality and time to pregnancy. 3. Quantify the degree of misclassification bias generated by retrospective exposure assessment methods in common use (participant self-report of past exposure using a recall questionnaire and/or archival environmental monitoring data) compared to prospective peri-ovulational biomonitoring and sampling. The proposal is to recruit a pre-conception cohort, conduct prospective exposure assessment via biomarkers and questionnaires, evaluate the association of exposure with time to pregnancy, and compare prospectively measured exposures with retrospectively estimated exposures. Collected biospecimens will be banked as a repository for future analyses. Creation of this cohort and specimen bank will launch the independent career of Principal Investigator (Early Stage) Porucznik.

描述（由申请人提供）：该提案将解决1）在人类发展的最早阶段的环境暴露的准确评估，以及2）这些暴露与试图构想的300对夫妇的社区居住在300对夫妇中的相关性。在动物模型和有限的流行病学研究中，选择用于测量，消毒副产品（DBP）和双酚A（BPA）的污染物与不良生殖结果有关。通过传统的流行病学手段（例如，使用问卷调查或怀孕早期的环境监测）（例如在第一次产前就诊时，或生态措施，例如从饮用水系统监测中报告的污染物水平分配），接触这些和其他环境污染物的暴露并未通过传统的流行病学手段进行很好的衡量。生物标志物可以允许对内剂量进行更精确的估计，但是对于瞬时暴露和半衰期的化学物质，必须在适当的时间收集生物标本，以便与关键发育窗口相关的个人暴露。如果暴露是短暂的，或者相关化学物质的半衰期短，则在怀孕早期收集的生物标志物可能为时已晚，无法检测到孕期暴露。在这种类型的设计中，也会错过影响受孕概率的卵石周围暴露，因为未能受孕的夫妻不会进入产前护理。具体目的1。使用一种新型的环境和生物学标本的自我收集策略，通过在人类的前观察群中进行前瞻性，卵外生物监测（DBP）（DBP）和BIS-苯酚A（BPA），该策略是通过观察到社区居住人群中生育迹象而触发的环境和生物学标本的新型策略。 2。评估前瞻性测量的男性和女性暴露之间的关联与精液质量和怀孕时间的生殖结果之间的关联。 3。与前瞻性的卵周生物监测和抽样相比，通过回顾性暴露评估方法（参与者使用召回问卷和/或档案环境监测数据）产生的错误分类偏差程度（参与者自我报告和/或档案环境监测数据）。该提案是招募概念前队列，通过生物标志物和问卷进行前瞻性暴露评估，评估暴露与妊娠时间的关联，并与前瞻性测量的暴露与回顾性估计的暴露进行了比较。收集的生物测量将作为未来分析的存储库。创建此队列和标本库将启动主要研究员（早期）Porucznik的独立职业。

项目成果

The Home Observation of Periconceptional Exposures (HOPE) study, a prospective cohort: aims, design, recruitment and compliance.

• DOI: 10.1186/s12940-016-0153-9
• 发表时间: 2016-06-08
• 期刊: Environmental health : a global access science source
• 作者: Porucznik CA;Cox KJ;Schliep KC;Wilkins DG;Stanford JB
• 通讯作者: Stanford JB

Exposure Classification and Temporal Variability in Urinary Bisphenol A Concentrations among Couples in Utah--The HOPE Study.

• DOI: 10.1289/ehp.1509752
• 发表时间: 2016-04
• 期刊: Environmental health perspectives
• 影响因子: 10.4
• 作者: Cox KJ;Porucznik CA;Anderson DJ;Brozek EM;Szczotka KM;Bailey NM;Wilkins DG;Stanford JB
• 通讯作者: Stanford JB

A Preliminary Study of Biomonitoring for Bisphenol-A in Human Sweat.

人体汗液中双酚 A 生物监测的初步研究。

• DOI: 10.1093/jat/bkv055
• 发表时间: 2015
• 期刊: Journal of analytical toxicology
• 影响因子: 2.5
• 作者: Porucznik,ChristinaA;Cox,KyleyJ;Wilkins,DianaG;Anderson,DavidJ;Bailey,NicoleM;Szczotka,KathrynM;Stanford,JosephB
• 通讯作者: Stanford,JosephB

Decreased fecundity and sperm DNA methylation patterns.

• DOI: 10.1016/j.fertnstert.2015.09.013
• 发表时间: 2016-01
• 期刊: Fertility and sterility
• 影响因子: 6.7
• 作者: Jenkins TG;Aston KI;Meyer TD;Hotaling JM;Shamsi MB;Johnstone EB;Cox KJ;Stanford JB;Porucznik CA;Carrell DT
• 通讯作者: Carrell DT