Multifunction High Throughput System for Neurophysiology of Behavior

行为神经生理学多功能高通量系统

• 批准号: 8781844
• 负责人: DONALD JAY WOODWARD
• 金额: $ 49.99万
• 依托单位: BIOGRAPHICS, INC.
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-15 至 2017-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8781844
• 关键词: Adopted; Adoption; Alcohol abuse; Alcohol consumption; Alcohol dependence; Alcoholism; Alcohols; Amplifiers; Animal Behavior; Animals; Behavior; Behavior Control; Behavioral; Behavioral Research; Brain; Brain region; Chemical Stimulation; Chemicals; China; Client; Cognition; Collection; Communication; Complex; Computer software; Computers; Data; Data Analyses; Data Set; Detection; Development; Devices; Disease; Dopamine; Drug Addiction; Drug Evaluation; Drug abuse; Drug effect disorder; Electrochemistry; Electronics; Equipment; Ethanol; Evaluation Research; Evolution; Family; Funding; Future; Genetic Models; Goals; Head; Housing; Image; Infusion Pumps; Infusion procedures; Ingestion; Intake; Intravenous infusion procedures; Investments; Laboratories; Lasers; Lead; Life; Light; Liquid substance; Measurement; Measures; Memory; Microdialysis; Mission; Motor; Movement; Mus; National Institute of Drug Abuse; National Institute of Mental Health; National Institute of Neurological Disorders and Stroke; National Institute on Alcohol Abuse and Alcoholism; Neurons; Neurosciences; Optics; Pain; Performance; Peripheral; Pharmaceutical Preparations; Phase; Phenotype; Physiologic pulse; Population; Procedures; Psyche structure; Pump; Rattus; Research; Research Activity; Rodent; Scanning; Schizophrenia; Seizures; Self Administration; Services; Signal Transduction; Site; Sleep; Small Business Innovation Research Grant; Source; Speed; Staging; Stimulus; Syringes; System; Systems Development; Technology; Testing; Therapeutic; Therapeutic Agents; Time; Tissues; Tube; Wireless Technology; Work; advanced system; aging brain; alcohol measurement; alcohol research; alcohol testing; analog; base; carbon fiber; commercialization; cost effective; cost effectiveness; data acquisition; design; digital; dopaminergic neuron; drinking; drug of abuse; experience; improved; innovation; instrument; instrumentation; mesolimbic system; miniaturize; neurophysiology; new technology; novel; parallel processing; prototype; public health relevance; research study; response; sensor; sensory gating; subcutaneous; validation studies

DESCRIPTION (provided by applicant): The goal of this project is to create a system to conduct research with the assistance of a 'High Throughput Multifunction System for Neurophysiology of Behavior'. This Phase II application to the Omnibus Solicitation is based on the Phase I for PA-08-001 that was funded by NIAAA 5R43AA020676-02. The advanced system will provide capabilities for many other Behavioral Neurophysiology research activity related to the missions of NIAAA, NIMH, NINDS, NIDA, and NIA. Multiple new technologies will be integrated to achieve a high performance high throughput system so that single operator can conduct multiple experiments. The main focus is to design and assemble an advanced data acquisition and control module for multiple continuous long duration studies of animals living in an experimental chamber. A rotating platform with tethered instrumentation will follow the movements of the animal. The purpose of the arrangement is to avoid the need for multiple swivel connection that limits the connections of different types that can be made with a moving animal. This arrangement will allow multiple electrical, optical and fluid connections to be made over long times. Installation of laser light sources and syringe infusion pumps on the rotating platform is a novel design. An advance data acquisition electronics module for recording and stimulation from a large number of channels will consist of an Altera Arria V GX FPGA with a PCIe connection to a PC motherboard. The multiple functions will allow continuous analog signal recording spike waveform capture, simultaneously electrochemical detection from many channels. The module also includes extensive capabilities for multiple imager support. The device is re-purposed from a DARPA that supported 'Gigapixel' camera project to enable parallel processing from hundreds of imager devices. The unique design will enable assembly of very large system configurations 10,000 or more parallel channels for future neuroscience as related technology matures. The compact unit will be installed above an experimental chamber on a unit that will rotate by a motor system triggered by movement of the rodent. The rotating system has been designed for transfer of power and control. High speed wireless, optical, or digital communication from the rotating unit will allow data and control signals to pass from each experimental chamber to a host computer data acquisition service A further aim is to advance a universal real time state machine for control of behavioral chambers and multiple devices for sensing and stimulation. Remote control client software will be created for control and analysis of data. An additional aim is to conduct evaluation studies of drugs related to therapy for multiple applications in behavioral neurophysiology related to development of therapeutic agents. Neuron population activity and multiple carbon fibers will employ fast scan electrochemistry to detect dopamine release transient in multiple brain regions with rats housed continuously over thirty days while testing responses. Microdialysis probes, multiple precision syringe pumps, camera imaging, and a colorimetric system will be adapted for repeated measurement of tissue alcohol levels in rats ingesting ethanol over time with intermittent access. Efficiency of microdialysis research will be enhanced by allowing use of multiple probes along with an improved automated collection system. A group of Collaborators to validate will provide critical and essential tests and evaluation for research applications in alcohol and drug addiction, schizophrenia, aging brain, sleep and brain states, pain modulation, and seizure prediction. The overall goal of the introduction of the interrelated enhancements is to reduce the required labor and instrumentation to achieve high efficiency for many types of behavioral research conducted simultaneously in multiple chambers, while employing a family of sensors and technologies that will be adapted for use with the rotating system. By achieving a high level of integration and efficiency the system aims to result in a System for Development of Therapeutic Drugs and Procedures'

描述（由申请人提供）：该项目的目的是在“高吞吐量多功能系统中用于行为的神经生理学”的协助下创建一个系统来进行研究。该II期应用于综合招标是基于由NIAAA 5R43AA020676-02资助的PA-08-001的I期。高级系统将为许多其他行为神经生理学研究活动提供与NIAAA，NIMH，NINDS，NIDA和NIA的任务有关的能力。将集成多种新技术以实现高性能高通量系统，以便单个操作员可以进行多个实验。 主要重点是设计和组装高级数据采集和控制模块，以对生活在实验室中的动物进行多种连续的长时间研究。带有束缚仪器的旋转平台将遵循动物的运动。安排的目的是避免需要多种旋转连接，以限制可以用动物进行的不同类型的连接。这种布置将允许长时间进行多种电气，光学和流体连接。在旋转平台上安装激光光源和注射器输液泵是一种新颖的设计。 用于录制和刺激的预先数据采集电子模块将包括一个Altera Arria V GX FPGA，并与PCIPE连接到PC主板。多个功能将允许连续的模拟信号记录尖峰波形捕获，同时从许多通道进行电化学检测。该模块还包括多个成像器支持的广泛功能。该设备是从支持“ Gigapixel”相机项目的DARPA中重新使用的，以从数百个成像器设备中实现并行处理。独特的设计将使非常大的系统配置组装10,000或更多的并行通道，以使未来的神经科学作为相关的技术成熟。紧凑的单元将安装在一个实验室上方，该单元将通过啮齿动物的运动触发的电机系统旋转。 旋转系统已设计用于传输功率和控制。旋转单元的高速无线，光学或数字通信将允许数据和控制信号从每个实验室传递到主机计算机数据采集服务的进一步目标是推进通用的实时状态机器，以控制行为室和多个设备以感知和刺激。将创建遥控客户端软件以控制和分析数据。 另一个目的是对与治疗剂开发有关的行为神经生理学多种应用的药物进行评估研究。神经元种群活性和多种碳纤维将采用快速扫描电化学来检测多巴胺释放的瞬态，在测试反应的同时，在三十天内，大鼠连续饲养大鼠。微透析探针，多个精确的注射泵，相机成像和比色系统将被调整，以重复测量随着时间的流逝，随着时间的推移，随着时间的推移摄入乙醇的组织酒精含量。通过允许使用多个探针以及改进的自动收集系统，将提高微透析研究的效率。一组验证的合作者将为酒精和药物成瘾，精神分裂症，大脑衰老，睡眠和大脑状态，疼痛调节和癫痫发作预测的研究应用提供关键和基本的测试和评估。 引入相互关联的增强的总体目标是减少 需要劳动和仪器以在多个室内同时进行的许多类型的行为研究中实现高效率，同时采用了一个传感器和技术系列，这些传感器和技术将适用于旋转系统。通过实现高水平的整合和效率，系统旨在导致制定治疗药物和程序的系统。