Multifunction High Throughput System for Neurophysiology of Behavior

行为神经生理学多功能高通量系统

• 批准号: 8006362
• 负责人: DONALD JAY WOODWARD
• 金额: $ 29.9万
• 依托单位: BIOGRAPHICS, INC.
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-15 至 2013-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8006362
• 关键词: Achievement; Alcohol abuse; Alcoholism; Algorithms; Animal Behavior; Animals; Antipsychotic Agents; Area; Behavior; Behavior Control; Behavioral; Brain; Brain Injuries; Brain Pathology; Brain region; Caliber; Client; Cognition; Communication; Complex; Computer software; Computers; Data; Data Collection; Data Set; Databases; Development; Disease; Drug Evaluation; Drug abuse; Drug effect disorder; Electronics; Epilepsy; Equipment; Evaluation; Evaluation Research; Future; Genetic Models; Goals; Housing; Individual; Infusion Pumps; Infusion procedures; Intravenous infusion procedures; Laboratories; Lead; Life; Liquid substance; Longitudinal Studies; Memory; Motor; Movement; Mus; National Institute of Drug Abuse; National Institute of Mental Health; National Institute of Neurological Disorders and Stroke; National Institute on Alcohol Abuse and Alcoholism; Neurons; Optics; Output; Pain; Performance; Pharmaceutical Preparations; Phase; Physiology; Population; Procedures; Protocols documentation; Psyche structure; Pump; Rattus; Recovery; Recovery of Function; Research; Rodent; Schizophrenia; Seizures; Services; Signal Transduction; Sleep; Sleep Disorders; Small Business Innovation Research Grant; Sorting - Cell Movement; Speed; Stimulus; Syringes; System; Technology; Testing; Therapeutic; Therapeutic Agents; Time; Tube; Wireless Technology; Work; advanced system; aging brain; base; brain research; computerized data processing; cost; cost effective; cost effectiveness; data acquisition; design; digital; experience; high throughput technology; indexing; innovation; instrument; instrumentation; interstitial; mange; neurophysiology; new technology; novel; prototype; relating to nervous system; relational database; research study; response; sensor

DESCRIPTION (provided by applicant): The goal of this project is to create a system to conduct 'High Throughput Multifunction System for Neurophysiology of Behavior', in response to PA-08-001. The advanced system will provide capabilities for many other Behavioral Neurophysiology research applications for NIMH, NINDS, NIDA, NIAAA, and NIA. Multiple new technologies will be integrated to achieve a high performance high throughput system. A group of Collaborators will provide critical test and evaluation for research applications in schizophrenia, aging brain, sleep and brain states, pain modulation, seizure prediction, and alcohol abuse. A First Aim is to design and assemble a data acquisition and control module for continuous long duration studies of animals living in an experimental chamber. The planned arrangement will also allow multiple drug infusions in long term studies of therapeutic drugs. A version of a new compact low cost multichannel recording/stimulation and spike sorting electronics module will be designed based on an advanced field programmable gate array. The compact unit will be installed above an experimental chamber on a unit that will rotate by a motor system triggered by movement of the rodent. High speed wireless, optical, or digital communication from the rotating unit will allow data and control signals to pass from each experimental chamber to a host computer data acquisition service. Multichannel electrical, optical stimulation and electrochemical sensing will be integrated in efficient manner. The tethered cabling from the data acquisition system will be inserted through a large diameter aperture to the mouse or rat. Tethered instrumentation will rotate with the animal and thus allow long duration studies, as has been routine in sleep research on rodents. Installation of syringe infusion pumps on the unit that rotates with the animal is a novel design allowing for multiple fluid connections to and from the animals without use of fluid or optical swivels. This design eliminates the traditional commutator system with many expensive slip rings, and allows greatly expanded numbers of wired, semi-wireless, fluidic, and optical connections to be made with a moving animal over long times. A Second Aim is to create remote control client software to control both an existing spike sorter software module and a real time state machine module for experimental control of behavioral studies. This enhancement will allow a remote client with a single operator to control multiple workstations. A second part of this Aim is to begin use and evaluation of a data handling strategy that employs multiple time index files in parallel with an existing high speed database engine to contain all data types collected. Input and output functions will interface with Matlab scripts and templates to reduce the time required for analysis the large volumes of data. Many types of experimental control information, neurophysiological and anatomical data will be organized with use of the relational database engine. A Third Aim is to conduct evaluation studies of drugs related to therapy for multiple applications in behavioral neurophysiology related to development of therapeutic agents. Neuron population activity will be recorded in multiple brain regions with rats housed continuously over thirty days while testing responses. The overall goal of the introduction of the interrelated enhancements is to reduce the required labor and instrumentation to achieve high efficiency. The system will provide infinite expandability to mange either very large parallel recording system or multiple individual experimental chambers. This procedure will fully test basic features of the new technology developed and conduct a key proof of concept evaluation for a wide range of application in behavioral neurophysiology. PUBLIC HEALTH RELEVANCE: This project will develop an extremely efficient and cost effective instrument to control and test a wide range of behavior of animals while recording neural activity of multiple brain regions. The system includes a number of enhancements of both electronic instrumentation and the extensive software needed to enable high throughput studies. Such a system is needed to determine the actions of drugs that can cause amelioration of schizophrenia, aging brain, sleep disorder, epilepsy, alcohol and drug abuse and recovery from brain injury. The system will useful for efficient studies related to drug abuse, alcoholism, and improvement of memory, cognition in mental disease, and function in aging brain

描述（由申请人提供）：该项目的目的是为了响应PA-08-001，创建一个系统来执行“行为神经生理学的高吞吐量多功能系统”。高级系统将为NIMH，NINDS，NIDA，NIAAA和NIA的许多其他行为神经生理学研究应用提供功能。将集成多种新技术以实现高性能高通量系统。一组合作者将为精神分裂症，大脑衰老，睡眠和大脑状态，疼痛调节，癫痫发作预测和酗酒的研究应用提供重要的测试和评估。 第一个目的是设计和组装数据采集和控制模块，以对生活在实验室中的动物进行持续的长时间研究。计划的安排还将允许在治疗药物的长期研究中进行多次药物输注。新的紧凑型低成本多通道记录/刺激和尖峰排序电子模块的版本将基于高级现场可编程门阵列。紧凑的单元将安装在一个实验室上方，该单元将通过啮齿动物的运动触发的电机系统旋转。旋转单元的高速无线，光学或数字通信将允许数据和控制信号从每个实验室传递到主机计算机数据采集服务。多通道电气，光学刺激和电化学传感将以有效的方式整合。来自数据采集系统的束缚电缆将通过大量直径孔插入到小鼠或大鼠。束缚仪器将与动物一起旋转，因此可以进行长时间的研究，这是对啮齿动物的睡眠研究的常规研究。在用动物旋转的设备上安装注射器输液泵是一种新颖的设计，允许在不使用液体或光学旋转的情况下与动物进行多种流体连接。这种设计消除了带有许多昂贵的滑环的传统换向器系统，并允许长时间使用移动的动物进行大量的有线，半无线，流体和光学连接。 第二个目的是创建远程控制客户端软件，以控制现有的Spike Sorter软件模块和用于行为研究实验控制的实时状态机模块。这种增强功能将使带有单个操作员的远程客户端可以控制多个工作站。此目的的第二部分是开始使用和评估数据处理策略，该策略与现有的高速数据库引擎并行多个时间索引文件一起包含收集的所有数据类型。输入和输出功能将与MATLAB脚本和模板接口，以减少分析大量数据所需的时间。通过使用关系数据库引擎，将组织许多类型的实验控制信息，神经生理和解剖学数据。 第三个目的是对与治疗剂开发有关的行为神经生理学多种应用的药物进行评估研究。神经元种群活性将记录在多个大脑区域，其中大鼠在三十天内连续饲养，同时测试反应。 引入相互关联的增强功能的总体目标是减少所需的劳动力和工具以实现高效率。该系统将为管理非常大的平行记录系统或多个单独的实验室提供无限的可扩展性。该过程将充分测试开发的新技术的基本特征，并为行为神经生理学中广泛应用的概念评估的关键证明。 公共卫生相关性：该项目将开发一种非常有效且具有成本效益的工具，以控制和测试动物的广泛行为，同时记录多个大脑区域的神经活动。该系统包括电子仪器的许多增强功能和实现高通量研究所需的广泛软件。需要这样的系统来确定可能导致精神分裂症，大脑衰老，睡眠障碍，癫痫，酒精和药物滥用以及脑损伤恢复的药物的作用。该系统将有助于与药物滥用，酒精中毒以及记忆，精神疾病认知以及衰老大脑的功能有关的有效研究