Leica TCS SP8 FSU AOBS 405 UV Spectral Confocal Microscope

Leica TCS SP8 FSU AOBS 405 紫外光谱共焦显微镜

• 批准号: 8640318
• 负责人: Akiko Nishiyama
• 金额: $ 45.63万
• 依托单位: UNIVERSITY OF CONNECTICUT STORRS
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-05-01 至 2015-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8640318
• 关键词: Arts; Biology; Communities; Confocal Microscopy; Connecticut; Data Collection; Development; Dyes; Ensure; Event; Faculty; Fees; Floor; Funding; Funding Applicant; Hour; Housing; Image; Lasers; Microscope; Minor; Modeling; Molecular; Neurobiology; Neurosciences; Nuclear; Peer Review; Pharmacy facility; Physiology; Publications; Research; Research Personnel; Science; System; Time; United States National Institutes of Health; Universities; college; falls; fluorophore; instrument; member; programs; tool

DESCRIPTION (provided by applicant): Funds are requested for the purchase of a Leica TCS SP8 AOBS 405 UV Spectral Confocal Microscope to be used in the PNB Confocal Facility in the Department of Physiology and Neurobiology at the University of Connecticut, Storrs. The Department currently has a Leica TCS SP2 Spectral Confocal Microscope, which was purchased in 2001 with funds from NIH (1S10RR015684-01). The instrument is maintained as a departmental facility that is open to the entire Storrs campus community. During the past eleven years, it has been heavily used, averaging 7 hours a day, resulting in 73 peer-reviewed publications by four to eight NIH-funded major users. In 2006 Leica stopped manufacturing this model, and some parts are no longer available. There is not enough capacity on the existing confocal microscopes on the Storrs campus to accommodate the needs of the current users of the SP2 system in the event that a critical part cannot be replaced. Even if the existing instrument were to remain functional, many of the users critically need a 405 nm laser line to image the nuclear dye DAPI, along with the other fluorophores, but a 405 nm laser cannot be added to our existing SP2 unit. The other existing microscope on this campus with this capability is housed in a separate building and is too heavily used to accommodate our users' needs. The user group has grown from four NIH-funded investigators at the time of the original application in 2000 to eight major users and two minor users, and we have two new faculty members who will join our department this fall, whose research heavily depends on confocal microscopy. Confocal microscopy has now become an indispensable tool for the research program of the users in our department and a standard tool in cellular, molecular, and developmental neuroscience. Thus, there is a pressing need to acquire a new confocal system to ensure that the current users of the existing SP2 system have uninterrupted access to a functional confocal microscope and avoid delays in data collection and publication. The new confocal microscope will be housed in the center of the first floor of the Pharmacy Biology Building where the department is housed. It will be maintained by user fees and additional support from the Department, the Dean of the College of Liberal Arts and Sciences, and the Office of Vice President for Research.

描述（由申请人提供）：要求资金购买LEICA TCS SP8 AOBS 405紫外光谱共聚焦显微镜，用于在康涅狄格大学Storrs的生理学和神经生物学系的PNB共聚焦设施中使用。该部门目前拥有Leica TCS SP2光谱共聚焦显微镜，该显微镜于2001年以NIH的资金（1S10RR015684-01）购买。该工具被维持在整个Storrs校园社区开放的部门设施。在过去的十一年中，它已经大量使用，每天平均7个小时，从而使73个同行评审的出版物由4至8个NIH资助的主要用户。 2006年，莱卡（Leica）停止制造此模型，而某些零件不再可用。在Storrs校园的现有共聚焦显微镜上没有足够的容量来满足SP2系统当前用户的需求，如果无法更换关键部分。即使现有的仪器保持功能，许多用户也需要405 nm激光线与其他荧光团以及其他405 nm激光器以及我们现有的SP2单元添加405 nm激光器。该校园中具有该能力的另一个现有显微镜位于单独的建筑物中，并且太过用来满足我们用户的需求。用户组从2000年原始申请的四名资助的调查人员开始发展，到八个主要用户和两个未成年人用户，我们有两个新的教职员工将在今年秋天加入我们的部门，他们的研究在很大程度上取决于共聚焦显微镜。现在，共聚焦显微镜已成为我们部门用户研究计划的必不可少的工具，并且是细胞，分子和发育神经科学领域的标准工具。因此，迫切需要购买新的共聚焦系统，以确保现有SP2系统的当前用户不间断地访问功能共聚焦显微镜，并避免在数据收集和出版物中延迟。新的共聚焦显微镜将安置在该部门所在的药房生物学大楼一楼的中心。它将通过用户费用和部门，文科和科学学院的院长以及研究副总裁办公室维护。

项目成果

The bHLH-PAS transcriptional complex Sim:Tgo plays active roles in late oogenesis to promote follicle maturation and ovulation.

bHLH-PAS 转录复合物 Sim:Tgo 在卵子发生晚期发挥积极作用，促进卵泡成熟和排卵。

• DOI: 10.1242/dev.201566
• 发表时间: 2023
• 期刊: Development (Cambridge, England)
• 作者: Oramas,Rebecca;Knapp,ElizabethM;Zeng,Baosheng;Sun,Jianjun
• 通讯作者: Sun,Jianjun

Direct reprogramming of oligodendrocyte precursor cells into GABAergic inhibitory neurons by a single homeodomain transcription factor Dlx2.

• DOI: 10.1038/s41598-021-82931-9
• 发表时间: 2021-02-11
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Boshans LL;Soh H;Wood WM;Nolan TM;Mandoiu II;Yanagawa Y;Tzingounis AV;Nishiyama A
• 通讯作者: Nishiyama A

Borrelia burgdorferi Co-Localizing with Amyloid Markers in Alzheimer's Disease Brain Tissues.

• DOI: 10.3233/jad-215398
• 发表时间: 2022
• 期刊: Journal of Alzheimer's disease : JAD
• 作者: Senejani AG;Maghsoudlou J;El-Zohiry D;Gaur G;Wawrzeniak K;Caravaglia C;Khatri VA;MacDonald A;Sapi E
• 通讯作者: Sapi E

NADPH oxidase-generated reactive oxygen species in mature follicles are essential for Drosophila ovulation.

• DOI: 10.1073/pnas.1800115115
• 发表时间: 2018-07-24
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Li W;Young JF;Sun J
• 通讯作者: Sun J