Michigan Lupus Epidemiology & Surveillance (MILES) Program Longitudinal Cohort

密歇根狼疮流行病学

• 批准号: 8718778
• 负责人: Emily Catherine Somers
• 金额: $ 75万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-30 至 2016-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8718778
• 关键词: Michigan; Lupus; Epidemiology; Surveillance; MILES

DESCRIPTION (provided by applicant): Project Summary/Abstract This proposal is to extend the Michigan Lupus Epidemiology & Surveillance (MILES) Program beyond the nearly completed public health surveillance phase to a research phase, by creating and maintaining the MILES Longitudinal Cohort & Biobank, which will include both lupus cases from the existing surveillance registry and population-based controls. The overarching goal will be to prospectively collect data and biospecimens that will allow for comprehensive investigations related to the clinical course of lupus, including risk factors for both the onset and progression of disease and its comorbidities. Detailed data collection for all participants will include a number of measures related to sociodemographics, health/wellness, physical functioning, environmental exposures, genetics, and for female participants, a number of items related to women's health. Lupus-specific measures, including disease activity indices, will be assessed for the SLE patients. The biobank will include serum, plasma, PBMCs, DNA (isolated both from unfractionated PBMCs and lymphocyte subsets), hair and toenail clippings. Vaginal swabs for female participants of reproductive age will be collected, allowing for HPV DNA profiling. Three distinct research modules related to lupus etiology and comorbidities are integrated, with the topics specifically dealing with epigenetics, HPV infection, and centrally-mediated pain. Biobank specimens will be stored in appropriate conditions to allow for genotyping and future investigation of biomarkers of both disease and exposure. An emphasis will be placed on providing the capability to perform contemporary immunoassays, including DNA methylation of relevant lymphocyte subsets. Lastly, this proposal will support the performance of single nucleotide polymorphisms (SNP) genotyping using the ImmunoChip. The MILES Cohort will thus serve as a robust platform for ongoing and future research investigations that are relevant to the full spectrum of lupus and its complications.

描述（由申请人提供）：项目摘要/摘要该建议是将密歇根狼疮流行病学和监视（MILES）计划扩展到近乎完成的公共卫生监视阶段，通过创建和维护Miles纵向纵向共同的同胞和生物库来扩展到研究阶段，其中包括来自现有的Surveillance Contry Contry Contry Contress Contress Contry和Masused Contrance Surveillance Contrys and Masuesterance Contryss and Masusecters Contryss and Mases Laster Consects and casters。总体目标是前瞻性收集数据和生物测量，这将允许与狼疮的临床过程进行全面研究，包括疾病发作和进展及其合并症的风险因素。所有参与者的详细数据收集将包括许多与社会人口统计学，健康/健康，身体功能，环境暴露，遗传学和女性参与者有关的措施，这些措施与女性健康有关。将对SLE患者评估狼疮特异性的措施，包括疾病活动指数。生物库将包括血清，血浆，PBMC，DNA（从未分离的PBMC和淋巴细胞亚群中分离出来），头发和脚趾甲夹。将收集针对生殖年龄的女性参与者的阴道拭子，从而允许HPV DNA分析。与狼疮病因和合并症有关的三个不同的研究模块已整合，主题专门涉及表观遗传学，HPV感染和中央介导的疼痛。生物库标本将在适当的条件下存储，以允许对疾病和暴露的生物标志物进行基因分型和未来研究。将重点放在提供执行当代免疫测定的能力上，包括相关淋巴细胞亚群的DNA甲基化。最后，该提案将支持使用免疫化学的单核苷酸多态性（SNP）基因分型的性能。因此，Miles队列将成为与狼疮及其并发症及其并发症相关的持续研究和未来研究调查的强大平台。