Michigan Lupus Epidemiology & Surveillance (MILES) Program Longitudinal Cohort

密歇根狼疮流行病学

• 批准号: 8236581
• 负责人: Emily Catherine Somers
• 金额: $ 75万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-30 至 2016-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8236581
• 关键词: Michigan; Lupus; Epidemiology; Surveillance; MILES

DESCRIPTION (provided by applicant): Project Summary/Abstract This proposal is to extend the Michigan Lupus Epidemiology & Surveillance (MILES) Program beyond the nearly completed public health surveillance phase to a research phase, by creating and maintaining the MILES Longitudinal Cohort & Biobank, which will include both lupus cases from the existing surveillance registry and population-based controls. The overarching goal will be to prospectively collect data and biospecimens that will allow for comprehensive investigations related to the clinical course of lupus, including risk factors for both the onset and progression of disease and its comorbidities. Detailed data collection for all participants will include a number of measures related to sociodemographics, health/wellness, physical functioning, environmental exposures, genetics, and for female participants, a number of items related to women's health. Lupus-specific measures, including disease activity indices, will be assessed for the SLE patients. The biobank will include serum, plasma, PBMCs, DNA (isolated both from unfractionated PBMCs and lymphocyte subsets), hair and toenail clippings. Vaginal swabs for female participants of reproductive age will be collected, allowing for HPV DNA profiling. Three distinct research modules related to lupus etiology and comorbidities are integrated, with the topics specifically dealing with epigenetics, HPV infection, and centrally-mediated pain. Biobank specimens will be stored in appropriate conditions to allow for genotyping and future investigation of biomarkers of both disease and exposure. An emphasis will be placed on providing the capability to perform contemporary immunoassays, including DNA methylation of relevant lymphocyte subsets. Lastly, this proposal will support the performance of single nucleotide polymorphisms (SNP) genotyping using the ImmunoChip. The MILES Cohort will thus serve as a robust platform for ongoing and future research investigations that are relevant to the full spectrum of lupus and its complications.

描述（由申请人提供）：项目摘要/摘要该提案旨在通过创建和维护 MILES 纵向队列和生物库，将密歇根狼疮流行病学和监测 (MILES) 计划从即将完成的公共卫生监测阶段扩展到研究阶段，其中将包括现有监测登记处和基于人群的控制中的狼疮病例。总体目标是前瞻性地收集数据和生物样本，以便对狼疮临床病程进行全面调查，包括疾病发生和进展及其合并症的危险因素。所有参与者的详细数据收集将包括与社会人口统计、健康/保健、身体机能、环境暴露、遗传学相关的一系列措施，而对于女性参与者，则包括与妇女健康相关的许多项目。将为 SLE 患者评估狼疮特异性指标，包括疾病活动指数。该生物库将包括血清、血浆、PBMC、DNA（从未分级的 PBMC 和淋巴细胞亚群中分离出来）、头发和脚趾甲碎片。将收集育龄女性参与者的阴道拭子，以便进行 HPV DNA 分析。整合了与狼疮病因和合并症相关的三个不同的研究模块，主题专门涉及表观遗传学、HPV 感染和中枢介导的疼痛。生物库标本将储存在适当的条件下，以便进行基因分型以及未来对疾病和暴露生物标志物的研究。重点将放在提供执行当代免疫测定的能力，包括相关淋巴细胞亚群的 DNA 甲基化。最后，该提案将支持使用免疫芯片进行单核苷酸多态性 (SNP) 基因分型。因此，MILES 队列将成为一个强大的平台，用于正在进行和未来与狼疮及其并发症的全谱相关的研究调查。