CONFOCAL MICROSCOPY AND IMAGE ANALYSIS CORE

共焦显微镜和图像分析核心

• 批准号: 8358065
• 负责人: Xavier Alvarez-Hernandez
• 金额: $ 5.78万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8358065
• 关键词: Cells; Color; Computer software; Confocal Microscopy; Data; Data Analyses; Detection; Dimensions; Equipment; Funding; Grant; Hour; Human Resources; Image; Image Analysis; Immunohistochemistry; Individual; Institution; Journals; Label; Lasers; Life; Light; Microscope; Microscopy; National Center for Research Resources; Preparation; Primates; Principal Investigator; Publications; Research; Research Infrastructure; Research Personnel; Resources; Running; Scanning; Scientist; Services; Source; Spottings; System; Training; United States National Institutes of Health; base; cost; in vivo

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This Core provides state-of-the-art confocal microscopy, multilabel fluorescent labeling and detection, and image analysis support to every Division in the TNPRC, and to numerous affiliate research scientists at several institutions. The Core has a Leica TCS SP2 laser scanning confocal microscope system equipped with 3 lasers, with 6 laser lines available, capable of simultaneously collecting information in four channels (3 fluorescent and one for differential interference contrast). The system is attached to two microscopes; an upright (DMRE) and an inverted (DMIRE2), that allow for confocal microscopy of fixed preparations and also living cells. We have a separate workstation to run the Leica software for the analysis of the data collected. We also have Volocity Software for rendering data in 3 dimensions. The Image analysis system for transmitted light is based in the Leica DMRE microscope, a Spot camera and the latest ImagePro software in a dedicated workstation for investigators use in image analysis. We acquired a Nuance multi-spectral camera from CRI and the complementary Inform software for Image analysis. We are using the Nuance camera to capture and analyze images from immunohistochemistry. The multispectral capability of this camera allows us to separate the colors of individual labels better than ever before. In the last year four new users were trained to use the confocal microscope by themselves. In addition, core personnel assisted less trained users in 150 timeslots for 600 hours. The core performed 300 imaging services, including in vivo imaging and 2 or 3 color microscopy that generated more than 400 gigabytes of data. We have trained 3 users in image analysis with the Inform software but together with the core manager generated 500 GB in less than a month. This new piece of equipment is well used and is providing good results. The core directly contributed to 13 new publications two of which were featured on the cover of the journal.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 该核心为 TNPRC 的每个部门以及多个机构的众多附属研究科学家提供最先进的共焦显微镜、多标记荧光标记和检测以及图像分析支持。 Core 配备 Leica TCS SP2 激光扫描共焦显微镜系统，配备 3 个激光器，可提供 6 条激光线，能够同时收集 4 个通道（3 个荧光通道和 1 个用于微分干涉对比）的信息。 该系统附有两台显微镜；直立式 (DMRE) 和倒置式 (DMIRE2)，可对固定制剂和活细胞进行共聚焦显微镜检查。我们有一个单独的工作站来运行徕卡软件来分析收集的数据。我们还有用于渲染 3 维数据的 Volocity 软件。透射光图像分析系统基于 Leica DMRE 显微镜、Spot 相机和专用工作站中的最新 ImagePro 软件，供研究人员在图像分析中使用。我们从 CRI 购买了一台 Nuance 多光谱相机和用于图像分析的补充 Inform 软件。我们使用 Nuance 相机捕获和分析免疫组织化学图像。该相机的多光谱功能使我们能够比以往更好地分离各个标签的颜色。去年，四名新用户接受了自行使用共焦显微镜的培训。 此外，核心人员在 150 个时段为训练较少的用户提供了 600 个小时的帮助。 该核心执行 300 项成像服务，包括体内成像和 2 或 3 色显微镜，生成超过 400 GB 的数据。 我们使用 Inform 软件对 3 位用户进行了图像分析培训，但与核心管理人员一起在不到一个月的时间内生成了 500 GB 的数据。 这台新设备使用良好，效果良好。该核心直接贡献了 13 篇新出版物，其中两篇登上了该杂志的封面。