CONFOCAL MICROSCOPY AND IMAGE ANALYSIS CORE

共焦显微镜和图像分析核心

• 批准号: 7716248
• 负责人: Xavier Alvarez-Hernandez
• 金额: $ 2.5万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-21 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7716248
• 关键词: Arts; Cells; Color; Computer Retrieval of Information on Scientific Projects Database; Computer software; Confocal Microscopy; Data; Data Analyses; Depth; Detection; Dimensions; Fluorochrome; Funding; Grant; Hour; Human Resources; Image; Image Analysis; Institution; Label; Lasers; Length; Life; Light; Microscope; Microscopy; Preparation; Publications; Research; Research Personnel; Resolution; Resources; Running; Scanning; Scientist; Services; Source; Spottings; System; Time; Training; United States National Institutes of Health; Width; base; in vivo

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This Core provides state-of-the-art confocal microscopy, multilabel fluorescent labeling and detection, and image analysis support to every Division in the TNPRC, and to numerous affiliate research scientists at several institutions. The Core has a Leica TCS SP2 laser scanning confocal microscope system equipped with 3 lasers, with 6 laser lines available, capable of simultaneously collecting information in four channels (3 fluorescent and one for differential interference contrast). The system is attached to two microscopes an upright (DMRE) and an inverted (DMIRE2), that allow for confocal microscopy of fixed preparations and also living cells. We have a separate workstation to run the Leica software for the analysis of the data collected. We also have Volocity Software for the rendition of the data in 3 dimensions. The Image analysis system for transmitted light is based in the Leica DMRE microscope, a Spot camera and the latest ImagePro software in a dedicated workstation for investigators use in image analysis. The confocal system offers such benefits as: a) Multi-dimensional imaging, as it is possible to obtain images in four dimensions, length (x axis), width (y axis), depth (z axis) and time (t). b) Resolution improvement. c) Contrast improvement. d) Multicolor imaging. Several fluorochromes can be imaged at the same time. In the last year five new users were trained to use the confocal microscope by themselves. In addition, core personnel assisted less trained users in 145 timeslots for 580 hours. The core performed 470 imaging services, including in vivo imaging and 2 or 3 color microscopy that generated more than 200 gigabytes of data. The core directly contributed to 9 new publications.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 中心，不一定是研究者的机构。 该核心为 TNPRC 的每个部门以及多个机构的众多附属研究科学家提供最先进的共焦显微镜、多标记荧光标记和检测以及图像分析支持。 Core 配备 Leica TCS SP2 激光扫描共焦显微镜系统，配备 3 个激光器，可提供 6 条激光线，能够同时收集 4 个通道（3 个荧光通道和 1 个用于微分干涉对比）的信息。 该系统连接到两个显微镜，一个是正置显微镜（DMRE），一个是倒置显微镜（DMIRE2），可以对固定制剂和活细胞进行共聚焦显微镜检查。我们有一个单独的工作站来运行徕卡软件来分析收集的数据。我们还有 Volocity 软件用于以 3 维方式呈现数据。透射光图像分析系统基于 Leica DMRE 显微镜、Spot 相机和专用工作站中的最新 ImagePro 软件，供研究人员在图像分析中使用。共焦系统具有以下优点： a) 多维成像，因为可以获得四个维度的图像：长度（x 轴）、宽度（y 轴）、深度（z 轴）和时间（t）。 b) 分辨率提高。 c) 对比度改善。 d) 多色成像。 可以同时对多种荧光染料进行成像。 去年，五名新用户接受了自行使用共焦显微镜的培训。 此外，核心人员在 145 个时段为训练较少的用户提供了 580 小时的帮助。 该核心执行 470 项成像服务，包括体内成像和 2 或 3 色显微镜，生成超过 200 GB 的数据。 该核心直接贡献了9篇新出版物。