NEUTROPENIA FOLLOWING G-CSF ADMINISTRATION

G-CSF 给药后中性粒细胞减少症

• 批准号: 8358172
• 负责人: Xavier Alvarez-Hernandez
• 金额: $ 3.72万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8358172
• 关键词: Antibodies; Antigens; Blood Circulation; CSF3 gene; Confocal Microscopy; Endothelium; Funding; Grant; Label; Lung; Myelogenous; National Center for Research Resources; Neutropenia; Nuclear; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Source; Splenic Tissue; Staining method; Stains; United States National Institutes of Health; caveolin 1; cost; histiocyte; monocyte; neutrophil

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Shortly following G-CSF administration there is a dramatic yet transient disappearance of neutrophils from the circulation. The transient loss of neutrophils from the circulation following G-CSF administration appeared to be due to an accumulation of neutrophils within the pulmonary and splenic tissues. Detailed confocal microscopy using a myeloid/histiocyte antigen clone Mac387 which labels neutrophils and monocytes, an endothelial marker anti-caveolin-1-Cy3 antibody, and a nuclear stain (To-Pro3) showed that the neutrophils were elongated, appeared activated, and were tightly adhered to the endothelium of the pulmonary vasculature. These results suggest that the transient neutropenia following G-CSF administration is associated with an accumulation of neutrophils within the pulmonary and splenic tissues.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 G-CSF给药后不久，循环中的中性粒细胞急剧但短暂地消失。 G-CSF给药后循环中中性粒细胞的短暂损失似乎是由于肺和脾组织内中性粒细胞的积累所致。使用标记中性粒细胞和单核细胞的骨髓/组织细胞抗原克隆 Mac387、内皮标记物抗 Caveolin-1-Cy3 抗体和核染色 (To-Pro3) 进行详细的共聚焦显微镜检查，结果显示中性粒细胞被拉长、出现激活并被激活。与肺血管内皮细胞紧密粘附。这些结果表明，G-CSF给药后短暂的中性粒细胞减少与肺和脾组织内中性粒细胞的积累有关。