Comparative Medicine Infectious Diseases- Bethesda

比较医学传染病-贝塞斯达

• 批准号: 8745804
• 负责人: William Elkins
• 金额: $ 2989.08万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8745804
• 关键词: Aerobic; Affect; American; Animals; Assisted Reproductive Technology; Authorship; Beds; Bioterrorism; Breeding; Caring; Clinical; Communicable Diseases; Confounding Factors (Epidemiology); Cryopreservation; Data; Disease; Distress; Embryo; Emerging Communicable Diseases; Environment; Equipment; Exclusion; Experimental Designs; Food; Germ-Free; Gnotobiotic; Histopathology; Human; Hypersensitivity; Infectious Diseases Research; Journals; Knowledge; Laboratory Animal Medicine; Laboratory Animal Science; Laboratory Animals; Laws; Manuscripts; Medicine; Mission; Modification; Mus; National Institute of Allergy and Infectious Disease; Organism; Pain; Pathogenesis; Pathology; Preparation; Principal Investigator; Publications; Publishing; Regulation; Research; Research Activity; Research Personnel; Research Support; Resources; Services; Specialist; Technical Expertise; Techniques; Tissue Banks; Transgenic Mice; Veterinary Pathology; Virus; Work; air filter; assisted reproduction; biosafety level 3 facility; comparative; cost; design; experience; medical specialties; member; microorganism; occupational health/safety; programs; research study; sperm cell; tissue preparation

The CMB Infectious Disease Pathogenesis Section assisted Principal Investigators with experimental design, tissue collection and preparation, interpretation of histopathology findings, and assistance with manuscript preparation. CMB Research Support Specialists also provided technical expertise and support to NIAID Principal Investigators, assisting them with true cage to beachside support. Both pathology and technical proficiency resulted in numerous co-authorship opportunities. The CMB has completed the challenge of bringing a gnotobiotic facility on line. The gnotobiotic facility consists of 12 bio-exclusion units designed to keep the mice from becoming colonized with any adventitious microorganisms. Germ-free mice are free of all aerobic and anaerobic organisms with the possible exception of endogenous viruses. Breeding colonies and mice on study are maintained in semi-rigid isolators provided with HEPA-filtered air and autoclaved food, bedding, and supplies. Strict SOPs are followed to maintain the mice in a germ-free state. The CMB has an active Assisted Reproduction Technologies and Cryopreservation unit. Last year the ARTiC group had 68 sperm cryo request for 23 PIs and 2 requests for 1 PI inconjunction with Taconic. This year, the section had 30 requests from 14 PIs with 5 reuqeusts still in the pipeline. Last year, there were 40 Embryo Cryo requests from 14 PIs that resulted in 6607 empryos. There was 1 Taconic project from 1 PI that resulted in 312 embryos. This year, there were 8 requests from 8 PIs that resulted in 946 embryos and 2 Taconic projects that resulted in 240 embryos. There are 4 projects still in the pipeline for FY13. Last year there were 30 rederivation requests, this year there were 18. ARTiC ahd 3 requests for Transgenic mice in 2012 and 1 request in 2013. A mission of the Comparative Medicine Branch (CMB) is to provide support to the emerging and re-emerging infectious disease research conducted within the NIAID/DIR Animal Biosafety Level (ABSL) 3 facilities. Members of the Comparative Medicine Branch (CMB) along with a Division of Occupational Health and Safety (DOHS) representative have submitted a manuscript to the Journal of the American Association for Laboratory Animal Science (JAALAS) providing a background of laws, regulations, and an overview of programmatic requirements pertaining to the use of Select Agents. The manuscript was published in JAALAS in 2012.

CMB感染性疾病发病机理部分协助首席研究人员对实验设计，组织收集和制备，组织病理学发现的解释以及手稿准备的帮助。 CMB研究支持专家还为NIAID的首席研究人员提供了技术专长和支持，为他们提供了真正的笼子来提供海滨支持。 病理学和技术水平都带来了许多共同创作的机会。 CMB已经完成了将gnotobiotic设施置于线路的挑战。 gnotobiotic设施由12个生物排斥单元组成，旨在防止小鼠与任何不定的微生物殖民。无菌小鼠不含所有有氧和厌氧生物，但可能的内源性病毒除外。研究的繁殖菌落和小鼠在半刚毛的隔离器中保持了hepa过滤的空气和高压灭菌的食物，床上用品和供应。遵循严格的SOP将小鼠保持在无菌状态。 CMB具有主动的辅助繁殖技术和冷冻保存单元。 去年，Artic Group提出了68个精子冷冻要求，要求23个PI，并要求与Taconic提出1次PI结合的要求。 今年，该部分有14个PI的30条请求，其中5个Reuqeusts仍在管道中。 去年，14个PI的40个胚胎冷冻要求导致了6607个Empryos。 1个PI的Taconic项目产生了312个胚胎。 今年，有8个PIS的8个请求产生了946个胚胎和2个Taconic项目，产生了240个胚胎。 2013财年仍有4个项目。 去年有30个重新启动请求，今年有18个。122012的转基因小鼠的AHD 3请求和2013年的1个请求。比较医学分支（CMB）的任务是为新兴和重新出现的传染病研究提供支持，在NIAID/DIR DIRE AIMAL AIMAL AIMAL BIOSAFETY水平（ABSL）3设施中提供了支持。 比较医学分支机构（CMB）的成员以及职业健康与安全部（DOHS）代表已将手稿提交给美国实验室动物科学协会杂志（JAALAS）杂志，该杂志提供了法律，法规，法规的背景以及与选择者使用有关的程序要求的概述。 该手稿于2012年在Jaalas出版。