Comparative Medicine Infectious Diseases- Bethesda

比较医学传染病-贝塞斯达

• 批准号: 8946629
• 负责人: William Elkins
• 金额: $ 3221.79万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8946629
• 关键词: Aerobic; Affect; Air; Animals; Assisted Reproductive Technology; Authorship; Beds; Bioterrorism; Breeding; Caring; Clinical; Communicable Diseases; Confounding Factors (Epidemiology); Cryopreservation; Data; Disease; Distress; Emerging Communicable Diseases; Environment; Equipment; Exclusion; Experimental Designs; Food; Germ-Free; Gnotobiotic; Histopathology; Human; Hypersensitivity; Infectious Diseases Research; Knowledge; Laboratory Animal Medicine; Laboratory Animals; Manuscripts; Medicine; Mission; Modification; Mus; National Institute of Allergy and Infectious Disease; Organism; Pain; Pathogenesis; Pathology; Preparation; Principal Investigator; Publications; Research; Research Activity; Research Personnel; Research Support; Resources; Services; Specialist; Technical Expertise; Techniques; Tissue Banks; Veterinary Pathology; Virus; Work; assisted reproduction; biosafety level 3 facility; comparative; cost; design; experience; high efficiency particulate air filter; medical specialties; microorganism; programs; research study; tissue preparation

The CMB Infectious Disease Pathogenesis Section assisted Principal Investigators with experimental design, tissue collection and preparation, interpretation of histopathology findings, and assistance with manuscript preparation. CMB Research Support Specialists also provided technical expertise and support to NIAID Principal Investigators, assisting them with true cage to benchside support. Both pathology and technical proficiency resulted in numerous co-authorship opportunities. The CMB has successfully maintained a gnotobiotic facility, which consists of 12 bio-exclusion units designed to keep the mice from becoming colonized with any adventitious microorganisms. Germ-free mice are free of all aerobic and anaerobic organisms with the possible exception of endogenous viruses. Breeding colonies and mice on study are maintained in semi-rigid isolators provided with HEPA-filtered air and autoclaved food, bedding, and supplies. Strict SOPs are followed to maintain the mice in a germ-free state. The CMB has an active Assisted Reproduction Technologies and Cryopreservation unit. A mission of the Comparative Medicine Branch (CMB) is to provide support to the emerging and re-emerging infectious disease research conducted within the NIAID/DIR Animal Biosafety Level (ABSL) 3 facilities.

CMB感染性疾病发病机理部分协助首席研究人员对实验设计，组织收集和制备，组织病理学发现的解释以及手稿准备的帮助。 CMB研究支持专家还为NIAID的首席研究人员提供了技术专长和支持，并协助他们真正的笼子提供了板凳支持。 病理学和技术水平都带来了许多共同创作的机会。 CMB成功地维护了一个gnotobiotic设施，该设施由12个生物排斥单元组成，旨在防止小鼠与任何不定的微生物殖民。无菌小鼠不含所有有氧和厌氧生物，但可能的内源性病毒除外。研究的繁殖菌落和小鼠在半刚毛的隔离器中保持了hepa过滤的空气和高压灭菌的食物，床上用品和供应。遵循严格的SOP将小鼠保持在无菌状态。 CMB具有主动的辅助繁殖技术和冷冻保存单元。 比较医学分支（CMB）的任务是为NIAID/DIR Animal Biosafety水平（ABSL）3设施中进行的新兴和重新出现的传染病研究提供支持。