Comparative Medicine Infectious Diseases- Bethesda

比较医学传染病-贝塞斯达

• 批准号: 8336730
• 负责人: William Elkins
• 金额: $ 1549.04万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8336730
• 关键词: Affect; American; Animals; Aortic Aneurysm; Assisted Reproductive Technology; Authorship; Autopsy; Bioterrorism; Brain; Breeding; Caring; Clinical; Communicable Diseases; Complex; Confounding Factors (Epidemiology); Cryopreservation; Data; Disease; Distress; Educational workshop; Embryo; Emerging Communicable Diseases; Environment; Equipment; Evaluation; Excision; Experimental Designs; Freezing; Gnotobiotic; Histopathology; Human; Hypersensitivity; Infectious Diseases Research; Journals; Knowledge; Laboratory Animal Medicine; Laboratory Animal Science; Laboratory Animals; Laboratory Personnel; Laws; Manuscripts; Medicine; Mission; Modification; Mouse Strains; Mus; National Institute of Allergy and Infectious Disease; Organ; Organism; Pain; Pathogenesis; Pathology; Preparation; Principal Investigator; Publications; Regulation; Research; Research Activity; Research Personnel; Research Support; Resources; Saguinus; Services; Specialist; Spinal Cord; Technical Expertise; Techniques; Thrombosis; Tissue Banks; Veterinarians; Veterinary Pathology; Work; assisted reproduction; biosafety level 3 facility; comparative; cost; design; experience; medical specialties; meetings; member; nonhuman primate; occupational health/safety; posters; programs; research study; sperm cell; tissue preparation

The CMB Infectious Disease Pathogenesis Section assisted Principal Investigators with experimental design, tissue collection and preparation, interpretation of histopathology findings, and assistance with manuscript preparation. CMB Research Support Specialists also provided technical expertise and support to NIAID Principal Investigators. Both pathology and technical proficiency resulted in numerous co-authorship opportunities. The CMB has undertaken the challenge of bringing a gnotobiotic facility on line and will initiate internal breeding. The CMB has an active Assisted Reproduction Technologies and Cryopreservation unit. Last year the ARTiC group froze 7922 embryos of 32 mouse lines from 12 different PIs. This year we have banked 3000 embryos of 15 lines and have 13 lines in the pipeline. Last year we froze the sperm of 25 mouse strains for 9 PIs, this year we froze the sperm of 42 lines for 21 different PIs. Last year we had 51 rederivations and we have 25 so far this year. The ARTic group will be conducting a sperm cryopreservation workshop for the local AALAS chapter and give a talk on cryopreservation during their annual meeting; we also are going to present a poster at the ISTT meeting next October. We have found spontaneous intracardiac thrombosis and aortic aneurysms in tamarins. A mission of the Comparative Medicine Branch (CMB) is to provide support to the emerging and re-emerging infectious disease research conducted within the NIAID/DIR Animal Biosafety Level (ABSL) 3 facilities. An example that illustrates the type of research support provided is the formation of the necropsy team. A team of CMB Veterinarians spent several months performing and perfecting numerous non human primate (NHP) necropsies in preparation to support a complex neurovirulent study. The study required the meticulous removal of brain and spinal cord from NHPs for evaluation by laboratory personnel. The necropsy team successfully performed all NHP necropsies including brain, spinal cord, and other organ removal for the entire study. Members of the Comparative Medicine Branch (CMB) along with a Division of Occupational Health and Safety (DOHS) representative have submitted a manuscript to the Journal of the American Association for Laboratory Animal Science (JAALAS) providing a background of laws, regulations, and an overview of programmatic requirements pertaining to the use of Select Agents. The manuscript is currently under review.

CMB感染性疾病发病机理部分协助首席研究人员对实验设计，组织收集和制备，组织病理学发现的解释以及手稿准备的帮助。 CMB研究支持专家还为NIAID首席研究人员提供了技术专长和支持。 病理学和技术水平都带来了许多共同创作的机会。 CMB提出了将gnotobiotic设施置于线路并将启动内部育种的挑战。 CMB具有主动的辅助繁殖技术和冷冻保存单元。 去年，Artic Group冻结了来自12种不同PI的32个小鼠线的7922个胚胎。今年，我们已经将3000个胚胎包含了15条线，并在管道中有13行。去年，我们冻结了9个PI的25个小鼠菌株的精子，今年我们冻结了21种不同PI的42行精子。去年，我们进行了51次重新培训，今年到目前为止我们有25种。 Artic Group将为当地AALAS分会举办精子冷冻保存研讨会，并在年度会议期间就冷冻保存进行演讲；我们还将在明年10月的ISTT会议上介绍海报。 我们已经发现雌酪蛋白的自发性心脏血栓形成和主动脉瘤。 比较医学分支（CMB）的任务是为NIAID/DIR Animal Biosafety水平（ABSL）3设施中进行的新兴和重新出现的传染病研究提供支持。 一个说明提供的研究支持类型的示例是尸检小组的形成。 一支CMB兽医花了几个月的时间表演并完善了许多非人类灵长类动物（NHP）尸检，以准备支持一项复杂的神经毒性研究。 该研究要求从NHP中细致地去除大脑和脊髓，以评估实验室人员。 尸检小组成功地完成了所有NHP尸检，包括大脑，脊髓和其他器官去除整个研究。 比较医学分支机构（CMB）的成员以及职业健康与安全部（DOHS）代表已将手稿提交给美国实验室动物科学协会杂志（JAALAS）杂志，该杂志提供了法律，法规，法规的背景以及与选择者使用有关的程序要求的概述。 手稿目前正在审查中。