cIEF of glycoproteins in short nanoporous channels

短纳米孔通道中糖蛋白的 cIEF

• 批准号: 8497635
• 负责人: MARY J. WIRTH
• 金额: $ 14.96万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2014-12-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8497635
• 关键词: Address; Automation; Biological Assay; Biological Markers; Boxing; Cancer Detection; Cancer Diagnostics; Cancer Patient; Charge; Clinical; Collaborations; Data; Defect; Detection; Devices; Diagnostic; Glycoproteins; Height; Human; Isoelectric Focusing; Label; Length; Malignant Neoplasms; Malignant neoplasm of prostate; Medicine; Methods; Miniaturization; Modeling; Myoglobin; NIH Program Announcements; Nanotechnology; National Cancer Institute; Oncologist; Patients; Prostate-Specific Antigen; Proteins; Publishing; Reproducibility; Resolution; Sampling; Sensitivity and Specificity; Serum; Serum Proteins; Sheep; Sialic Acids; Silicon Dioxide; Specificity; System; Technology; Testing; Thick; Vision; Width; Work; base; electric field; improved; miniaturize; nanopore; new technology; public health relevance; statistics

DESCRIPTION (provided by applicant): New technology to enable higher throughput in biomarker discovery is urgently needed. Medicine is rich in protein biomarker candidates for cancer, but poor in clinically validated biomarkers. The reason is that the methods for discovery are very low in throughput, usually giving false indications due to the statistics of small numbers Targeted searches of glycoforms are promising for the discovery of protein biomarkers for cancer. We propose to determine the feasibility of 3-mm channels packed with nonporous silica for rapid isoelectric focusing of the glycoforms of an immunoextracted protein from serum. These channels have a 96-well format for seamless integration into a fully automation analysis, from serum to detection, enabling thousands of serum samples per day to be characterized. Prostate specific antigen (PSA) is chosen as the model protein because there is published evidence that isoelectric focusing can resolve glycoforms with higher specificity for cancer, and there is a wealth of data and serum samples for this biomarker. We have established a collaboration with a clinical oncologist to obtain human serum samples from prostate cancer patients for testing the feasibility of this technology.

描述（由申请人提供）：迫切需要能够提高生物标志物发现吞吐量的新技术。 医学上有丰富的癌症候选蛋白质生物标志物，但临床验证的生物标志物却很少。 原因是发现方法的通量非常低，通常由于统计数据较少而给出错误指示。糖型的靶向搜索对于发现癌症蛋白质生物标志物很有希望。 我们建议确定填充无孔二氧化硅的 3 毫米通道用于从血清中免疫提取的蛋白质的糖型快速等电聚焦的可行性。这些通道采用 96 孔格式，可无缝集成到从血清到检测的全自动分析中，每天能够对数千个血清样本进行表征。 选择前列腺特异性抗原（PSA）作为模型蛋白，因为有已发表的证据表明等电聚焦可以解析对癌症具有更高特异性的糖型，并且该生物标志物有大量数据和血清样本。 我们与临床肿瘤学家建立了合作，从前列腺癌患者身上获取人体血清样本，以测试该技术的可行性。

项目成果

Silica colloidal crystals as emerging materials for high-throughput protein electrophoresis.

二氧化硅胶体晶体作为高通量蛋白质电泳的新兴材料。

• DOI: 10.1208/s12248-013-9506-2
• 发表时间: 2013
• 期刊: The AAPS journal
• 作者: Njoya,NadineK;Birdsall,RobertE;Wirth,MaryJ
• 通讯作者: Wirth,MaryJ