cIEF of glycoproteins in short nanoporous channels

短纳米孔通道中糖蛋白的 cIEF

• 批准号: 8497635
• 负责人: MARY J. WIRTH
• 金额: $ 14.96万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2014-12-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8497635
• 关键词: Address; Automation; Biological Assay; Biological Markers; Boxing; Cancer Detection; Cancer Diagnostics; Cancer Patient; Charge; Clinical; Collaborations; Data; Defect; Detection; Devices; Diagnostic; Glycoproteins; Height; Human; Isoelectric Focusing; Label; Length; Malignant Neoplasms; Malignant neoplasm of prostate; Medicine; Methods; Miniaturization; Modeling; Myoglobin; NIH Program Announcements; Nanotechnology; National Cancer Institute; Oncologist; Patients; Prostate-Specific Antigen; Proteins; Publishing; Reproducibility; Resolution; Sampling; Sensitivity and Specificity; Serum; Serum Proteins; Sheep; Sialic Acids; Silicon Dioxide; Specificity; System; Technology; Testing; Thick; Vision; Width; Work; base; electric field; improved; miniaturize; nanopore; new technology; public health relevance; statistics

DESCRIPTION (provided by applicant): New technology to enable higher throughput in biomarker discovery is urgently needed. Medicine is rich in protein biomarker candidates for cancer, but poor in clinically validated biomarkers. The reason is that the methods for discovery are very low in throughput, usually giving false indications due to the statistics of small numbers Targeted searches of glycoforms are promising for the discovery of protein biomarkers for cancer. We propose to determine the feasibility of 3-mm channels packed with nonporous silica for rapid isoelectric focusing of the glycoforms of an immunoextracted protein from serum. These channels have a 96-well format for seamless integration into a fully automation analysis, from serum to detection, enabling thousands of serum samples per day to be characterized. Prostate specific antigen (PSA) is chosen as the model protein because there is published evidence that isoelectric focusing can resolve glycoforms with higher specificity for cancer, and there is a wealth of data and serum samples for this biomarker. We have established a collaboration with a clinical oncologist to obtain human serum samples from prostate cancer patients for testing the feasibility of this technology.

描述（申请人提供）：迫切需要新技术以使生物标志物发现更高的吞吐量。 药物富含蛋白质生物标志物候选癌症，但在经过临床验证的生物标志物中较差。 原因是，发现方法的吞吐量非常低，通常由于较少的糖型搜索量的统计数据，可以给出错误的适应症，这对于发现蛋白质生物标志物的癌症有望。 我们建议确定用非孔二氧化硅填充的3毫米通道的可行性，以快速地从血清中免疫剥夺蛋白质的糖型糖剂。这些通道具有96孔格式，可将无缝集成到完全自动化分析中，从血清到检测，可以表征数千种血清样品。 将前列腺特异性抗原（PSA）选择为模型蛋白，因为有公开的证据表明，等电聚焦可以解决具有较高特异性癌症的糖型，并且该生物标志物有大量的数据和血清样品。 我们已经与临床肿瘤学家建立了合作，以从前列腺癌患者中获取人类血清样品，以测试该技术的可行性。

项目成果

Silica colloidal crystals as emerging materials for high-throughput protein electrophoresis.

二氧化硅胶体晶体作为高通量蛋白质电泳的新兴材料。

• DOI: 10.1208/s12248-013-9506-2
• 发表时间: 2013
• 期刊: The AAPS journal
• 作者: Njoya,NadineK;Birdsall,RobertE;Wirth,MaryJ
• 通讯作者: Wirth,MaryJ