Slip-flow chromatography

滑流色谱法

• 批准号: 8524926
• 负责人: MARY J. WIRTH
• 金额: $ 10万
• 依托单位: BIOVIDRIA, INC.
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-08-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8524926
• 关键词: Blood capillaries; Bovine Serum Albumin; Caliber; Chromatography; Column Chromatography; Diagnostic; Diffuse; Diffusion; Disease; Equilibrium; Legal patent; Length; Licensing; Liquid Chromatography; Marketing; Methods; Modification; Paper; Particle Size; Peptides; Phase; Preparation; Proteins; Research Personnel; Silicon Dioxide; Small Business Technology Transfer Research; Speed; Surface; Technology; Time; Universities; Width; base; capillary; design; improved; nano; nanofluidic; particle; pressure; public health relevance; success

DESCRIPTION: The product will be a packed capillary for nanoUHPLC of peptides that provides a dramatic increase in speed and efficiency. Current commercial columns for high speed and efficiency use sub-2 mm particles. The proposed technology uses sub-0.2 mm particles. The ability to use such small particles for UHPLC is enabled by the phenomenon of slip flow, which gives enhanced volume flow rates in reversed-phase liquid chromatography. Slip flow is a breakthrough that overcomes the fundamental limit to peak broadening caused by the parabolic velocity profile in the mobile phase. Using nonporous sub-0.2 mm particles also eliminates intraparticle diffusion while giving high phase ratios. Preliminary results show that sub micrometer nonporous silica particles reach the diffusion limit for efficiency in protein separations, and the diffusion limit is expected to be reached for the faster diffusing peptides. This gives plate numbers that are independent of length, allowing columns less than 2 cm in length to give 100,000 plates with high speed. The specific aims are to 1) optimize the particle diameter, 2) optimize the separation length, and 3) evaluate the nano LC-MS of a tryptic digest.

说明：该产品将是肽nanouhplc的包装毛细管，可急剧提高速度和效率。 高速和效率的当前商业柱使用低于2 mm的颗粒。所提出的技术使用低于0.2毫米的颗粒。 通过滑动流的现象可以使使用如此小的颗粒用于UHPLC的能力，从而增强了相反相相液相色谱的体积流量。 滑动流是一个突破，它克服了由流动阶段中抛物线速度曲线引起的峰值扩大的基本限制。 使用非孔子sub-0.2 mM颗粒也消除了颗粒内扩散，同时给出了高相比。 初步结果表明，亚微米非孔二氧化硅颗粒达到蛋白质分离效率的扩散极限，并且预计将达到更快的扩散肽的扩散极限。 这给出了独立于长度的板数，允许柱的长度小于2 cm，从而使100,000个板具有高速。 具体目的是1）优化颗粒直径，2）优化分离长度，3）评估胰蛋白酶消化的纳米LC-MS。