Insecticide Resistance in Anopheles minimus Mosquitoes

微小按蚊的杀虫剂抗药性

• 批准号: 8212246
• 负责人: Guiyun Yan
• 金额: $ 5.88万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-12-01 至 2013-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8212246
• 关键词: Accounting; Address; Adult; Advisory Committees; Africa South of the Sahara; African; Anopheles Genus; Anopheles gambiae; Anti-malarial drug resistance; Asia; Bacillus thuringiensis; Beds; Biological; Biological Assay; Breeding; Case Study; China; Chloroquine; Climate; Clinical; Cohort Studies; Communities; Culicidae; Data; Development; Diagnosis; Doctor of Philosophy; Drug Metabolic Detoxication; Ecology; Entomology; Epidemic; Epidemiology; Failure; Female; Foundations; Future; Genes; Genetic Polymorphism; Grant; Habitats; Incidence; Infection; Insecta; Insecticide Resistance; Insecticides; Kenya; Laboratories; Larva; Life; Linear Models; Malaria; Medical; Medical Entomology; Metabolic; Methods; Mixed Function Oxygenases; Modeling; Molecular; Monitor; Mosquito Control; Mutation; Nucleotides; Parasites; Parents; Phenotype; Plasmodium falciparum; Play; Population; Population Genetics; Postdoctoral Fellow; Public Health; Publishing; Pyrimethamine-Sulfadoxine; Regression Analysis; Research; Research Training; Residual state; Resistance; Role; Scientist; Seasons; Sentinel; Site; Southeastern Asia; Spatial Distribution; Statistical Models; Students; System; Temperature; Testing; Training; United States National Institutes of Health; Universities; World Health Organization; base; cost effective; design; land cover; land use; larval control; meter; parent grant; predictive modeling; public health relevance; pyrethroid; research study; resistance mechanism; resistance mutation; skills training; tool; transmission process; vector; vector control; vector mosquito

DESCRIPTION (provided by applicant): Malaria is a major public health problem in China. Pyrethroid-impregnated bed nets and indoor spray with pyrethroids are the main malaria vector control tools. However, the wide use of pyrethroids has resulted in the emergence and spread of resistance in mosquito populations. Insecticide resistance has significantly hindered the efforts of malaria control in China and worldwide. This application will focus on resistance mechanisms in an important malaria vector in Southern China, Anopheles minimus C. The central hypothesis is that both knockdown resistance (kdr) and metabolic detoxification confer resistance to pyrethroids in An. minimus, and consequently, the resistance level of a mosquito to pyrethroids is contingent on mutations in kdr gene and monooxygenase activity. The kdr gene polymorphism in natural populations will be determined, and correlations between the kdr mutations, monooxygenase activity with pyrethroid resistance phenotype will be examined. This research will play an important foundation for the development of molecular- or biochemically-based resistance diagnosis tools in this important malaria vector species in China and Southeast Asia. The proposed research will contribute to building research capacity in China by training one Ph.D. student and one postdoc fellow and strengthen the molecular entomology laboratory at the Nanjing Medical University. This research will be conducted as an extension of NIH grant R01 AI050243-07. PUBLIC HEALTH RELEVANCE: This application focuses on resistance mechanisms in the most important malaria vector in Southern China, Anopheles minimus C. Understanding the resistance mechanisms will play an important foundation for the development of new resistance diagnosis tools. Further, this research will contribute to building research capacity in medical entomology in China by providing research training for one Ph.D. student and one postdoc fellow, and strengthen the molecular entomology laboratory in Nanjing Medical University.

描述（由申请人提供）：疟疾是中国的一个主要公共卫生问题。浸渍拟除虫菊酯的蚊帐和拟除虫菊酯室内喷雾是主要的疟疾病媒控制工具。然而，拟除虫菊酯的广泛使用导致蚊子群体中耐药性的出现和蔓延。杀虫剂耐药性严重阻碍了中国和世界范围内的疟疾控制工作。本申请将重点研究中国南方一种重要的疟疾病媒微小按蚊的耐药机制。中心假设是击倒抗性 (kdr) 和代谢解毒均赋予按蚊对拟除虫菊酯的抗性。最小，因此，蚊子对拟除虫菊酯的抗性水平取决于 kdr 基因和单加氧酶活性的突变。将测定自然群体中的kdr基因多态性，并检查kdr突变、单加氧酶活性与拟除虫菊酯抗性表型之间的相关性。这项研究将为中国和东南亚这一重要疟疾病媒物种开发基于分子或生化的耐药性诊断工具奠定重要基础。拟议的研究将通过培养一名博士来促进中国的研究能力建设。学生和一名博士后，并加强南京医科大学的分子昆虫学实验室。这项研究将作为 NIH 拨款 R01 AI050243-07 的延伸进行。 公共卫生相关性：本申请重点研究中国南方最重要的疟疾病媒微小按蚊的耐药机制。了解耐药机制将为开发新的耐药诊断工具奠定重要基础。此外，这项研究将通过为一名博士提供研究培训，有助于建设中国医学昆虫学的研究能力。学生和博士后一名，并加强南京医科大学分子昆虫学实验室。

项目成果

Permanent Genetic Resources added to Molecular Ecology Resources Database 1 April 2011-31 May 2011.

2011年4月1日至2011年5月31日将永久遗传资源添加到分子生态资源数据库。

• 发表时间: 2011-09
• 影响因子: 7.7
• 作者: Molecular Ecology Resources Primer Development Consortium;Bonizzoni, Mariangela;Bourjea, Jérôme;Chen, Bin;Crain, B J;Cui, Liwang;Fiorentino, V;Hartmann, S;Hendricks, S;Ketmaier, V;Ma, Xiaoguang;Muths, Delphine;Pavesi, L;Pfautsch, S;Rieger, M
• 通讯作者: Rieger, M