MAPP Research Networdk TIssue Analysis & Technology Core

MAPP 研究网络k 组织分析

• 批准号: 8137301
• 负责人: M. Scott Lucia
• 金额: $ 58.4万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-09 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8137301
• 关键词: Area; Biological Assay; Body Fluids; Collection; Computer Assisted; Core Facility; Diagnostic; Drug Kinetics; Enzyme-Linked Immunosorbent Assay; Gel Chromatography; Generations; Genomics; Genotype; Histopathology; Image; Image Analysis; Immunohistochemistry; Mass Spectrum Analysis; Microtomy; Oxidative Stress; Paraffin; Pathogenesis; Patients; Pelvic Pain; Performance; Process; Protein Array; Proteins; Proteome; Proteomics; RNA; Research; Resources; Reverse Transcriptase Polymerase Chain Reaction; Site; Specimen; Specimen Handling; Syndrome; Techniques; Technology; Tissue Microarray; Tissues; Woman; base; biobank; biological research; chronic pelvic pain; cytokine; insight; laser capture microdissection; men; metabolomics; morphometry; programs; response; success; two-dimensional

DESCRIPTION (provided by applicant): The overall aim of this application is to establish a Tissue Analysis and Technology Core (TATC) in support of the Multi-disciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) Research Network as described in RFA-DK-07-003. The MAPP Research Network will utilize a multi-site and multi-disciplinary approach to provide new insights into the pathogenesis and treatment of Chronic Pelvic Pain Syndromes, and establish a new and valuable biorepository resource for current and future research efforts. In response to the RFA, the TATC will interact with all research entities in the MAPP Network and provide expertise and resources in the areas of: 1) Biorepository Collection, Management and Distribution for all body fluid and tissue specimens generated by the MAPP Discovery Sites, and the generation of specialized assay platforms from these specimens such as protein and/or tissue arrays, DMA or RNA extractions as needed for Discovery Site efforts or the Ancillary Projects Program; 2) Histopathology and Tissue Morphometry including diagnostic interpretation, paraffin processing and microtomy, cryomicrotomy, laser capture microdissection, immunohistochemistry, and computer-assisted imaging/ image analysis; 3) Proteomics/ Metabolomics by providing a state-of-the-art centralized mass spectrometry facility to assist with proper collection and handling of specimens, consultative assistance for proteomic and/or metabolomics studies, and performance of a wide variety of assays, including two-dimensional gel- and chromatography-based proteome profiling, protein arrays, cytokine arrays, multiplexed ELISA, RT-PCR, infrared imaging, mass spectrometry and NMR-based targeted and non-targeted metabolite profiling, pharmacokinetic studies, oxidative stress assessment, and mass spectrometry analyses; and 4) Genomics/ Genotyping by providing consultative assistance and a state-of-the-art genomics facility to assist with advanced genotyping techniques. It is our underlying hypothesis that the quality, reliability and applicability of biological research on pelvic pain syndromes can only be guaranteed through the use of high quality non-biased patient specimens that have been handled, processed, analyzed and interpreted in a uniform manner. The MAPP Research Network will be the largest research initiative of its kind for chronic pelvic pain syndrome, a common but understudied condition in men and women. The establishment of a Tissue Analysis and Technology Core Facility is crucial to the success of this research program.

描述（由申请人提供）：本申请的总体目标是建立一个组织分析和技术核心（TATC），以支持慢性盆腔疼痛研究的多学科方法（MAPP）研究网络，如 RFA- 中所述DK-07-003。 MAPP 研究网络将利用多地点、多学科的方法，为慢性盆腔疼痛综合征的发病机制和治疗提供新的见解，并为当前和未来的研究工作建立一个新的、有价值的生物样本库资源。为了响应 RFA，TATC 将与 MAPP 网络中的所有研究实体互动，并提供以下领域的专业知识和资源： 1) MAPP 发现站点生成的所有体液和组织样本的生物样本库收集、管理和分发，根据发现站点工作或辅助项目计划的需要，从这些样本中生成专门的检测平台，例如蛋白质和/或组织阵列、DMA 或 RNA 提取； 2) 组织病理学和组织形态测定，包括诊断解释、石蜡处理和切片术、冷冻切片术、激光捕获显微切割、免疫组织化学和计算机辅助成像/图像分析； 3) 蛋白质组学/代谢组学，通过提供最先进的集中式质谱设施来协助正确收集和处理样本、蛋白质组学和/或代谢组学研究的咨询援助以及各种分析的执行，包括两种分析基于三维凝胶和色谱的蛋白质组分析、蛋白质阵列、细胞因子阵列、多重 ELISA、RT-PCR、红外成像、质谱和基于 NMR 的靶向和非靶向代谢物分析、药代动力学研究、氧化应激评估和质谱分析； 4) 基因组学/基因分型，提供咨询援助和最先进的基因组学设施，以协助先进的基因分型技术。我们的基本假设是，只有使用经过统一方式处理、处理、分析和解释的高质量、无偏见的患者标本，才能保证盆腔疼痛综合征生物学研究的质量、可靠性和适用性。 MAPP 研究网络将成为针对慢性盆腔疼痛综合征的此类研究中规模最大的一项研究计划，慢性盆腔疼痛综合征是一种男性和女性常见但尚未得到充分研究的疾病。组织分析和技术核心设施的建立对于该研究计划的成功至关重要。