BSL3 Flow Biomarker and Imaging

BSL3 流式生物标志物和成像

基本信息

批准号：
8437243
负责人：
Gregory D Sempowski
金额：
$ 32.82万
依托单位：
UNIV OF NORTH CAROLINA CHAPEL HILL
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8437243
关键词：
Animal Model Animals Applied Research Area Basic Science Biological Biological Assay Biological Markers Bioluminescence Blood Cell Separation Cells Collaborations Complete Blood Count Computer Workstations Containment Core Facility Development Diagnostic Emergency Situation Emerging Communicable Diseases Fee-for-Service Plans Flow Cytometry Fluorescence Funding Future Goals Hematology Host Defense Human Image Imaging Techniques Immune response Immunologic Monitoring Immunophenotyping Infection Institutes Investigation Laboratories Leadership Life Monitor National Institute of Allergy and Infectious Disease Natural Immunity Pathogenicity Preparation Procedures Protein Array Protein Array Analysis Qualifying Reader Research Activity Research Personnel Running Sampling Secure Services Specimen Technology Tissues Training Training Activity Universities Vaccines adaptive immunity base biodefense biothreat cost effective cost efficient drug development in vivo insight meetings member novel therapeutics operation pathogen research facility

项目摘要

The overall objective of this core proposal is to establish a safe, secure and comprehensive Biosafety Level - 3 (BSL3) host-pathogen interaction core to support and enhance the activities of the SERCEB/RCE investigators. The core will provide state-of-the-art flow cytometry and cell sorting, multi-modal live animal imaging, and multiplex biomarker analysis for SERCEB or other RCE investigators running studies in the Regional Biocontainment Laboratory at Duke or for member investigators that send specimens to the core for analysis. To accomplish this goal we have available four base technologies in BSL3 containment: a BDFACS Aria cell sorter, an IVIS-Xenogen Spectrum imager, a BibRad luminex bead array reader and an Abbott Cell Dyn 3700 veterinary hematology analyzer. In support of these base technologies we have offline-computer workstations outside containment, dedicated BSL2 and BSL3 laboratory space for sample preparation, highly trained technical staff in BSL3 and ABSL3 procedures and a PI and Co-investigator with an established track record in immune monitoring research and core facility management. These core technologies are contained at BSL3 in the NIAID-RBL at Duke and run as a core in collaboration with the proposed SERCEB Aerobiology/Animal Models core. This will allow comprehensive host-pathogen analysis at BSL3 under appropriate Standard Operating Procedures for SERCEB/RCE investigators. The use of flow cytometry and in vivo imaging techniques will allow us to monitor both host response and the course of infection, replication, persistence, and dissemination of priority pathogens. Blood and tissue biomarker analysis will provided further valuable insight into host-pathogen interactions which will only enhance the challenge and mechanistic studies proposed by projects in the SERCEB consortium. Areas of active biomedical investigation to be supported by this host-pathogen interation core include fundamental pathogenicity of Priority and Select Agent pathogens, innate and adaptive immunity and host'defense, and development of drugs, diagnostics and vaccines for identified bio-threat pathogens.

该核心提案的总体目标是建立安全、可靠和全面的生物安全水平—— 3 (BSL3) 宿主-病原体相互作用核心，支持和增强 SERCEB/RCE 的活动调查人员。该核心将提供最先进的流式细胞术和细胞分选、多模式活体动物为 SERCEB 或其他 RCE 研究人员进行成像和多重生物标志物分析杜克大学的区域生物防护实验室或供将样本发送到核心的成员研究人员使用进行分析。为了实现这一目标，我们在 BSL3 遏制中提供了四种基本技术：BDFACS Aria 细胞分选仪、IVIS-Xenogen Spectrum 成像仪、BibRad luminex 珠阵列读取器和 Abbott Cell Dyn 3700 兽用血液分析仪。为了支持这些基础技术，我们有安全壳外的离线计算机工作站、用于样品的专用 BSL2 和 BSL3 实验室空间准备、BSL3 和 ABSL3 程序方面训练有素的技术人员以及 PI 和联合研究员在免疫监测研究和核心设施管理方面拥有良好的记录。这些核心技术包含在杜克大学 NIAID-RBL 的 BSL3 中，并作为核心进行协作运行与拟议的 SERCEB 空气生物学/动物模型核心。这将允许全面的宿主病原体根据 SERCEB/RCE 调查人员的适当标准操作程序在 BSL3 进行分析。这使用流式细胞术和体内成像技术将使我们能够监测宿主反应和主要病原体的感染、复制、持久性和传播过程。血液和组织生物标志物分析将为宿主-病原体相互作用提供进一步有价值的见解，这只会加强 SERCEB 联盟项目提出的挑战和机制研究。领域该宿主-病原体相互作用核心支持的积极生物医学研究包括基本的优先和选择病原体的致病性、先天性和适应性免疫以及宿主防御，以及针对已识别的生物威胁病原体开发药物、诊断方法和疫苗。