Core 2: Biocontainment and Immune Monitoring Core

核心2：生物防护和免疫监测核心

基本信息

批准号：
10842500
负责人：
Gregory D Sempowski
金额：
$ 144.71万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-16 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10842500
关键词：
2019-nCoV Animal Model Animals Antibodies Biological Biological Assay Biological Markers COVID-19 pandemic Cell Separation Cells Collaborations Color Containment Coronavirus Development Diagnostic Disease Outbreaks Flow Cytometry Fluorescence-Activated Cell Sorting Goals Hamsters Hormones Human Immunize Immunologic Monitoring Immunology Immunophenotyping Infection Institution Laboratories Lead Leadership Lung Lavage Fluid Measures Mesocricetus auratus Microbiology Modeling Mus Pathogenicity Phenotype Plasma Procedures Proteins Quality Control Quantitative Reverse Transcriptase PCR Reader Reagent Recording of previous events Research Research Activity Research Personnel Resource Sharing Sampling Secure Serum Services Signal Transduction Sorting Speed Standardization Surface Technology Therapeutic United States National Institutes of Health Universities Vaccines Viral Viral Load result Virus animal coronavirus antibody-dependent cell cytotoxicity betacoronavirus betacoronavirus vaccine biocontainment facility biodefense chemokine cytokine cytotoxicity design expectation future outbreak instrument instrumentation laboratory module meetings nonhuman primate operation pathogenic virus programs quality assurance response transmission process vaccine development vaccine platform virology

项目摘要

Core 2: Biocontainment and Immune Monitoring Abstract The overall goal of this program is to develop a vaccine or vaccines that will be available when the next betaCoV outbreak occurs. In support of this overall goal, a Biocontainment and Immune Monitoring Core has been centralized at Duke. The Core is comprised of technologies and services offered through the Duke Regional Biocontanment Laboratory (Duke RBL), the Duke Human Vaccine Institute (DHVI) Flow Cytometry and Cell Sorting facility, and the Montefiori and Ferrari antibody characterization laboratories. The Duke RBL is one of 12 NIH-constructed RBLs, and was the first to begin operation in 2007. The Duke RBL provides an administrative umbrella for safe, secure and compliant BSL2-3/ABSL2-3 and Select Agent research activities at Duke University. The Duke RBL has state-of-the-art BSL3 biocontainment laboratory modules, high- containment cell sorting, a portfolio of service units (i. e. Microbiology, Virology, Immunology and Animal Models Support), an integrated ABSL3 vivarium to support small animal challenge models, and was purpose built to support the development of diagnostics, therapeutics and vaccines for emerging/reemerging infections and biodefense. The Core will routinely provide program investigators with high-containment cell sorting and comprehensive phenotyping, multiplex biomarker profiling, viral load, neutralization and antibody-dependent cellular cytotoxicity assays, and small animal SARS-CoV-2 challenge models. Critical to all five Core Aims/Tasks is a commitment to ongoing quality control of instruments and assays; and a strong commitment to anticipate and meet the needs of all four projects and the NHP Core. By educating and working in collaboration with program investigators we will optimize assays to meet their needs and more effectively utilize the state-of-the-art instrumentation and biocontainment facilities available through the Biocontaniment and Immune Monitoring Core. Comprehensive and centralized immune monitoring by a team with a proven track-record will add value to all proposed studies, and will allow project investigators to better, and more efficiently, design and develop panbetaCoV vaccines.

核心 2：生物防护和免疫监测抽象的该计划的总体目标是开发一种或多种疫苗，以便在下一次疫苗接种时可用。 β冠状病毒爆发。为了支持这一总体目标，生物防护和免疫监测核心已集中在杜克大学。核心由杜克大学提供的技术和服务组成区域生物防护实验室 (Duke RBL)、杜克人类疫苗研究所 (DHVI) 流式细胞术和细胞分选设施，以及 Montefiori 和 Ferrari 抗体表征实验室。公爵RBL 是 NIH 建立的 12 个 RBL 之一，第一个于 2007 年开始运营。杜克大学 RBL 提供安全、可靠、合规的 BSL2-3/ABSL2-3 和 Select Agent 研究活动的行政保护伞在杜克大学。杜克 RBL 拥有最先进的 BSL3 生物防护实验室模块、高遏制细胞分选，服务单位组合（即微生物学、病毒学、免疫学和动物学）模型支持），一个集成的 ABSL3 动物园，用于支持小动物挑战模型，其目的是旨在支持新发/复发感染的诊断、治疗和疫苗的开发和生物防御。核心将定期为项目研究人员提供高遏制细胞分选和全面的表型分析、多重生物标志物分析、病毒载量、中和和抗体依赖性细胞毒性测定和小动物 SARS-CoV-2 攻击模型。对所有五个核心都至关重要目标/任务是对仪器和检测的持续质量控制的承诺；和坚定的承诺预测并满足所有四个项目和 NHP 核心的需求。通过教育和工作与项目研究人员合作，我们将优化检测方法，更有效地满足他们的需求利用生物防护中心提供的最先进的仪器和生物防护设施和免疫监测核心。由经过验证的团队进行全面、集中的免疫监测跟踪记录将为所有拟议的研究增加价值，并使项目研究人员能够更好、更多有效地设计和开发 panbetaCoV 疫苗。