Immunology Core

免疫学核心

• 批准号: 7764322
• 负责人: Gregory D Sempowski
• 金额: $ 36.9万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-25 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7764322
• 关键词: Adjuvant; Antibodies; Antibody Formation; Area; Arts; Avidity; Biological; Biological Assay; Biological Markers; Biological Models; Blood; Cell Separation; Cells; Cellular Immunology; Development; Ensure; Enzyme-Linked Immunosorbent Assay; Exudate; Flow Cytometry; Frequencies; Goals; Gonorrhea; Hormones; Human; Immune; Immune response; Immunity; Immunization; Immunologic Monitoring; Immunology; Immunophenotyping; In Vitro; Inflammatory; Institutes; Investigation; Leadership; Modeling; Monitor; Mucosal Immunity; Mucous Membrane; Mus; Neisseria gonorrhoeae; Pathway interactions; Plasma; Protein Array; Proteins; Proteomics; Research; Research Personnel; Resource Sharing; Resources; Sampling; Services; Sexually Transmitted Diseases; Signal Transduction; Staining method; Stains; Surface Plasmon Resonance; Technology; Testing; Tissue Procurements; Treatment Protocols; Urine; Vaccination; Vaccine Adjuvant; Vaccines; Work; chemokine; cytokine; design; in vivo; insight; instrumentation; meetings; novel; pathogen; reconstitution; response; tissue culture

The overall objective of this Shared Resource Core is to provide comprehensive Immunology support to enhance the activities of the SE STI CRC investigators. The core will provide expertise in monitoring systemic and mucosal immunity with an emphasis on humoral, cellular and biomarker analyses. The Core will utilize in vivo immunization strategies, in vitro restimulation assays, state-of-the-art immunophenotyping and flow cytometry, ELISA and avidity (SPR) analysis of humoral responses, and multiplex inflammatory biomarker analysis. To accomplish Core goals the efforts and expertise of Dr. Sempowski's cellular immunology lab and Dr. Staats' mucosal immunology lab have been combined. Core leaders in addition have available to them the following Duke Human Vaccine Institute Shared Resources: Research Flow Cytometry, Proteomics-Biacore, and Immune Reconstitution. and Biomarker Analysis. These shared resources have the state-of-the-art instrumentation needed to provide worid-class immune monitoring to the SE STI CRC. The inclusion on an Immunology Core in the SE STI CRC will provide a common set of platforms for mechanistic analysis of host immune response for the SE STI CRC investigators. The use of in vitro restimulation assays, flow cytometry and ELISA/SPR will allow comprehensive host response monitoring to both challenge pathogens and experimental vaccines/adjuvants. Blood, tissue and culture supernatant inflammatory biomarker analysis will provide further valuable insight into specific host response pathways which will enhance the challenge and mechanistic studies proposed by center projects, as well as assist in identification of correlates of immunity for vaccination regimens developed by the SE STI CRC. Areas of active STI investigation to be supported by this Immunology Core include fundamental innate and adaptive immune responses (systemic and mucosal) to N. gonorrhoeae, and identification and development of novel adjuvants to augment induction of protective immunity against N. gonorrhoeae

该共享资源核心的总体目标是提供全面的免疫学支持 加强 SE STI CRC 调查员的活动。该核心将提供监控方面的专业知识 全身和粘膜免疫，重点是体液、细胞和生物标志物分析。核心 将利用体内免疫策略、体外再刺激测定、最先进的免疫表型分析 流式细胞术、ELISA 和体液反应的亲合力 (SPR) 分析以及多重炎症 生物标志物分析。为了实现核心目标，Sempowski 博士的细胞团队的努力和专业知识 免疫学实验室和Staats博士的粘膜免疫学实验室已合并。另外还有核心领导 为他们提供以下杜克人类疫苗研究所共享资源：研究流程 细胞计数、蛋白质组学-Biacore 和免疫重建。和生物标志物分析。这些共享的 资源拥有为患者提供世界级免疫监测所需的最先进的仪器 SE STI CRC。将免疫学核心纳入 SE STI CRC 将提供一套通用的 为 SE STI CRC 研究人员提供宿主免疫反应机制分析的平台。使用在 体外再刺激测定、流式细胞术和 ELISA/SPR 将实现全面的宿主反应 监测挑战病原体和实验疫苗/佐剂。血液、组织和培养 上清液炎症生物标志物分析将为特定宿主反应提供进一步有价值的见解 将加强中心项目提出的挑战和机制研究的途径，以及 协助识别 SE STI CRC 制定的疫苗接种方案的免疫相关性。 该免疫学核心支持的积极性传播感染研究领域包括基本的先天性和 对淋病奈瑟菌的适应性免疫反应（全身和粘膜）以及鉴定和发展 新型佐剂增强针对淋病奈瑟菌的保护性免疫的诱导