PROTEIN ENGINEERING OF BIRA FOR PROTEIN TAGGING

用于蛋白质标记的 BIRA 蛋白质工程

• 批准号: 8169295
• 负责人: CATHERINE L DRENNAN
• 金额: $ 0.41万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169295
• 关键词: Binding; Computer Retrieval of Information on Scientific Projects Database; E coli birA protein; Engineering; Funding; Grant; Institution; Mediating; Methods; Protein Engineering; Proteins; Research; Research Personnel; Resources; Source; United States National Institutes of Health; X-Ray Crystallography; improved

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To improve the efficiency of an E. coli Biotin Ligase (BirA) mediated protein tagging method, efforts are being made to increase the promiscuity of BirA by protein engineering of its binding pocket. Our contribution toward these engineering efforts uses X-ray crystallography to study the contacts between BirA and its substrates.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 为了提高大肠杆菌生物素连接酶 (BirA) 介导的蛋白质标记方法的效率，人们正在努力通过对其结合袋进行蛋白质工程来增加 BirA 的混杂性。我们对这些工程工作的贡献是使用 X 射线晶体学来研究 BirA 与其基底之间的接触。