BACTERIOPHAGE P22

噬菌体 P22

• 批准号: 8168526
• 负责人: Jonathan Alan King
• 金额: $ 4.3万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-15 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168526
• 关键词: Bacteriophage P22; Bacteriophages; Capsid; Computer Retrieval of Information on Scientific Projects Database; Funding; Genetic; Grant; Institution; Molecular Biology; Research; Research Personnel; Resolution; Resources; Salmonella Phages; Scaffolding Protein; Source; Structure; Tail; United States National Institutes of Health; Virus

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Bacteriophage P22, one of the best studied Salmonella phages, has been the object of tremendous genetic studies for molecular biology in the past several decades and is remarkable for its converting and transducting abilities. During the virus maturation into the mature phage, it starts as a precursor, called procapsid, with scaffolding proteins inside, then the dsDNA inserts into the expanding capsid shell via one of the twelve vertices, and scaffold proteins exit. The tail attaches to this vertex to become mature and infectious. Although both structures of P22 procapsid and mature phage had been solved to subnanometer (~9 Angstrom) resolution by cryo-EM since 2003 (Jiang W. et. al., Nat Struct Biol, 2003, 10:131-5.), most of structural details still remain elusive due to the low resolution.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 噬菌体 P22 是研究最深入的沙门氏菌噬菌体之一，在过去几十年中一直是分子生物学大量遗传学研究的对象，并以其转换和转导能力而著称。在病毒成熟为成熟噬菌体的过程中，它以称为原衣壳的前体开始，内部有支架蛋白，然后 dsDNA 通过 12 个顶点之一插入扩展的衣壳壳，支架蛋白退出。尾巴附着在这个顶点上变得成熟并具有感染力。尽管自 2003 年以来，P22 衣壳和成熟噬菌体的结构均已通过冷冻电镜解析至亚纳米（~9 埃）分辨率（Jiang W. et. al., Nat Struct Biol, 2003, 10:131-5.），但大多数由于分辨率较低，结构细节仍然难以捉摸。