BACTERIOPHAGE P22

噬菌体 P22

• 批准号: 8361056
• 负责人: Jonathan Alan King
• 金额: $ 4.9万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361056
• 关键词: Bacteriophage P22; Bacteriophages; Capsid; Electron Microscopy; Funding; Genetic; Grant; Molecular Biology; National Center for Research Resources; Principal Investigator; Research; Research Infrastructure; Resolution; Resources; Salmonella Phages; Scaffolding Protein; Source; Structure; Tail; United States National Institutes of Health; Virus; cost; macromolecule

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Bacteriophage P22, one of the best studied Salmonella phages, has been the object of tremendous genetic studies for molecular biology in the past several decades and is remarkable for its converting and transducting abilities. During the virus maturation into the mature phage, it starts as a precursor, called procapsid, with scaffolding proteins inside, then the dsDNA inserts into the expanding capsid shell via one of the twelve vertices, and scaffold proteins exit. The tail attaches to this vertex to become mature and infectious. Although both structures of P22 procapsid and mature phage had been solved to subnanometer (~9 ¿ ) resolution by cryo-EM since 2003 (Jiang W. et. al., Nat Struct Biol, 2003, 10:131-5.), most of structural details still remain elusive due to the low resolution.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供 该子项目的主要支持。 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源的子项目可能列出的总成本。 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 噬菌体 P22 是研究最深入的沙门氏菌噬菌体之一，一直是研究的对象 在过去的几十年里，分子生物学领域进行了大量的遗传学研究 在病毒成熟过程中其转化和转导能力非常出色。 成熟的噬菌体，它从称为原衣壳的前体开始，内部有支架蛋白，然后 双链DNA通过十二个顶点之一插入扩展的衣壳壳中，并且支架 尾巴附着在这个顶点上，变得成熟并具有传染性。 P22 原衣壳和成熟噬菌体的结构均已解析至亚纳米级（~9 ¿ ）自 2003 年以来通过冷冻电镜分辨率（Jiang W. et. al., Nat Struct Biol, 2003, 10:131-5.），大多数 由于分辨率较低，结构细节仍然难以捉摸。