Exploring the role of the central amygdala in vocalization

探索中央杏仁核在发声中的作用

• 批准号: 8522585
• 负责人: Ian Christopher Hall
• 金额: $ 3万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-04-01 至 2013-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8522585
• 关键词: Acoustics; Action Potentials; Address; Agonist; Amphibia; Amygdaloid structure; Aphasia; Asperger Syndrome; Auditory; Auditory system; Autistic Disorder; Behavior; Behavioral; Biological Assay; Biological Models; Brain; Brain region; Cells; Chemicals; Cognition Disorders; Communication; Communication impairment; Complex; Confocal Microscopy; Cues; Data; Dextrans; Disease; Disinhibition; Dyes; Electric Stimulation; Excision; Female; GABA Agonists; GABA Antagonists; Generations; Goals; Hearing; Impairment; Individual; Laryngeal Nerves; Lead; Lesion; Mammals; Maps; Measures; Midbrain structure; Motor Pathways; Neural Pathways; Neurons; Pathway interactions; Pattern; Play; Population; Preparation; Process; Production; Prosencephalon; Rana; Research; Research Training; Role; Sensory; Serotonin; Serotonin Agents; Serotonin Antagonists; Shapes; Signal Transduction; Social Behavior; Social Environment; Speech; Stimulus; Structure of terminal stria nuclei of preoptic region; Stuttering; Techniques; Thalamic structure; Voluntary Mutism; Xenopus laevis; autism spectrum disorder; base; dextran; extracellular; hindbrain; immunocytochemistry; inhibitor/antagonist; innovation; male; neural circuit; parabrachial nucleus; prevent; public health relevance; relating to nervous system; research study; response; reuptake; social; social cognition; social communication; tool; vocalization

DESCRIPTION (provided by applicant): The goal of my proposed research training is to explore the role of the amygdala in generating socially appropriate vocal responses to salient acoustic signals. The proposed experiments will identify the connections between the amygdala and the regions responsible for vocal production, the chemical messengers that convey signals along these pathways and the contributions of two distinct neural pathways to the initiation and shaping of vocal responses. Previous research demonstrates the importance of the amygdala in processing social stimuli but less is known about how this brain region generates the behavioral responses that are appropriate for a given social context. I will study this problem in a powerful model system (Xenopus laevis) in which vocal communication is well understood and the hindbrain vocal pattern generating circuit is mapped; distinctive fictive vocalizations can be evoked ex vivo facilitating neural circuit analyses. {{I have found that lesions of the central amygdala produce socially inappropriate vocal responses (as is the case for mammals), and that lesions of the bed nucleus of the stria terminalis produce deficits in the initiation of callig, frequently resulting in the lack of vocalization in response to social stimuli that normally induce calling. I will investigate the specific neural pathways involved in these two effects using neuroanatomical, electrophysiological and pharmacological techniques. The proposed experiments will address four specific aims. (1) Determine whether stimulation of the CeA is sufficient to induce answer calling. Electrical stimulation of the CeA will be used to initiate ficive answer calling from the excised brain (2) Identify the neurons in the CeA that project to the PbN and the BST. Dextran-conjugated dyes and confocal microscopy will determine which cells in the CeA project to the PbN and BST. Immunocytochemistry will determine whether they are GABAergic. (3) Determine whether disinhibition of PbN neurons is the mechanism through which the CeA produces answer calling. GABA agonists will be used to mimic input from the CeA in the isolated brain while recording fictive calling from the laryngeal nerve. (4) Determine whether stimulation of the BST initiates calling through a serotonergic mechanism. Serotonergic drugs will be used to block or facilitate fictive calling induced by electrical stimulation of the BST.}} By addressing these aims, this research will illuminate one of the fundamental functions of the amygdala, the generation of context-appropriate behavior. Further, it will contribute to a growing understanding of the role of the amygdala in social vocal behavior and could make important contributions to the treatment of communication disorders, such as speech aphasias, selective mutism, and stuttering, and social cognition disorders, such as Asperger's syndrome and autism spectrum disorders.

描述（由申请人提供）：我提出的研究培训的目的是探索杏仁核在产生社会适当的声音对显着声学信号的作用。提出的实验将确定杏仁核与负责人声生产的区域之间的联系，沿这些途径传达信号的化学信使以及两种不同的神经途径对声音反应的启动和塑造的贡献。先前的研究表明，杏仁核在处理社会刺激中的重要性，但对这个大脑区域如何产生适合给定社会环境的行为反应的了解较少。我将在强大的模型系统（Xenopus laevis）中研究这个问题，其中众所周知声音交流并绘制了后脑声模式产生电路；可以引起独特的虚拟发声，以促进神经回路分析。 {{{我发现中央杏仁核的病变会产生社交不适当的声音反应（与哺乳动物一样），并且质状末端的床核的病变会导致Callig的启动，通常导致缺乏对社会刺激的响应响应的声音。 打电话。我将使用神经解剖学，电生理和药理技术研究这两种效应所涉及的特定神经途径。 提出的实验将解决四个具体目标。 （1）确定CEA的刺激是否足以引起答案。 CEA的电刺激将用于发起来自切除的大脑的效率答案（2）识别CEA中的神经元将投影到PBN和BST。葡聚糖偶联的染料和共聚焦显微镜将确定CEA项目中哪些细胞到PBN和BST。免疫细胞化学将确定它们是否具有GABA能。 （3）确定PBN神经元的抑制是否是CEA产生答案的机制。 GABA激动剂将用于模仿孤立大脑中CEA的输入，同时记录喉神经的虚拟呼唤。 （4）确定BST的刺激是否通过血清素能机制引发。血清素能药物将通过解决这些目标来阻止或促进由BST的电刺激引起的虚构呼唤，这项研究将阐明杏仁核的基本功能之一，即适应环境的行为。此外，它将有助于对杏仁核在社会声音行为中的作用的越来越多，并可以为沟通障碍的治疗做出重要贡献，例如言语失语症，选择性叛变，口吃和社会认知障碍，例如asperger的综合征和自闭症谱系。