Multi-Scale Optical Imaging of the Cancer Vasculature and Tumor Microenvironment

癌症脉管系统和肿瘤微环境的多尺度光学成像

基本信息

批准号：
8564201
负责人：
Seok-Hyun Andy Yun
金额：
$ 21.28万
依托单位：
METHODIST HOSPITAL RESEARCH INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-28 至 2015-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8564201
关键词：
Abdomen Adhesions Anatomy Animal Experiments Animal Model Animals Architecture Area Back Biological Biological Process Biomechanics Biomedical Engineering Biomedical Technology Blood Blood Vessels Bone Marrow Boston Breathing Caliber Calvaria Carcinoma Catheters Cell membrane Cells Clinical Code Collaborations Colonoscopy Colorectal Polyp Confocal Microscopy Coronary Coronary artery Custom Descending colon Development Diagnostic Diagnostic Imaging Diffusion Disease Doctor of Philosophy Drug Delivery Systems Endoscopes Endothelium Epithelial Epithelium Esophageal mucous membrane Exocytosis Extracellular Matrix Extravasation Fiber Optics Floor Fluorescence Fluorescence Microscopy Frequencies Funding General Hospitals Generations Goals Hematopoietic Home environment Human Image Imaging Device Imaging Techniques Implant In Situ Individual Infiltration Inflammatory Investigation Journals Kinetics Knockout Mice Laboratories Laparoscopy Laparotomy Lasers Lead Length Life Light Location Longitudinal Studies Malignant Neoplasms Massachusetts Measures Mechanics Medicine Methodology Microscope Microscopic Microscopy Modification Molecular Monitor Morphologic artifacts Motion Mucous Membrane Mus Nature Neoplasms Normal Cell Operative Surgical Procedures Ophthalmology Optical Coherence Tomography Optics Organ Osteoblasts Outcome Paper Particle Size Pathology Patients Penetration Permeability Photons Physics Pilot Projects Postdoctoral Fellow Process Property Proto-Oncogene Protein c-kit Publishing Raman Spectrum Analysis Research Research Personnel Resolution Reticuloendothelial System Rice Rotation Sampling Sapphire Scanning Side Skin Source Speed Stem cells Structure Surface Surgical incisions Surveys System Techniques Technology Therapeutic Three-Dimensional Image Time Tissue Engineering Tissues Titania Titanium Triplet Multiple Birth Tube Tumor Biology Universities Vascular Endothelium animal facility base cell motility cell type design detector flexibility fluorophore gastrointestinal imaging graduate student high standard image processing imaging modality in vivo instrument instrumentation interest interstitial intestinal epithelium intravital microscopy lens light scattering medical schools minimally invasive multi-photon nanoparticle nanosized neoplastic cell new technology novel operation optical imaging photonics pressure professor research study response second harmonic shear stress stem tool tumor tumor microenvironment tumor progression two-photon viscoelasticity

项目摘要

Core 2 will provide the five projects with a number of unique advanced optical systems; collaborate with the project investigators in the design, execution, and analysis of animal experiments; and develop new instrumentation and methodology as needs arise. The Core will be established in Boston within the Wellman Center for Photomedicine at Massachusetts General Hospital (MGH), where the new techniques and pilot experiments will be carried out. However, routine intravital microscopy experiments will be carried out in Houston within the new CABIR building where ad-hoc facilities have been specifically designed. The Advanced Intravital Microscopy Core is lead by Dr. Yun with the collaboration of Dr. Richards-Kortum at Rice University and Dr. Lin. Dr. Yun (Ph.D. Physics) is an Assistant Professor at Harvard Medical School within the Wellman Center for Photomedicine of the Massachusetts General Hospital. The activity of the core will be co-developed in collaboration with Dr. Lin within the Wellman Center for Photomedicine Dr. Yun and Dr. Lin research groups comprise a total of about 20 postdoctoral fellows and graduate students, externally funded, and in the past 3 years have published papers in Nature, Nature Medicine, Nature Photonics, Blood, Journal of Experimental Medicine, as well as top optics journals. Dr. Richards-Kortum (Ph.D. Physics) is Stanley C. Moore Professor and chair of the Bioengineering Department at Rice University. The Advanced Intravital Microscopy Core will use its technological tools and expertise to (i) reconstruct the vascular architecture (diameter, length, tortuosity, permeability) and the hydrodynamic conditions (flow rate, shear stress at the wall) within the region of interest; (ii) to visualize the transport of nano- and micro-sized particles within the vasculature and tumor microenvironment measuring in particulat their rate of adhesion to the endothelium, rate of extravasation from the vascular compartment, rate of diffusion within the epithelium, rate of internalization and possible exocytosis from normal and tumor cells. The transport of nanoparticles will be analyzed using the miniature front-view probe via minimally-invasive laparotomy.

Core 2将为这5个项目提供多项独特的先进光学系统；与项目研究人员合作设计、执行和分析动物实验；并根据需要开发新的仪器和方法。该核心将设在波士顿马萨诸塞州总医院 (MGH) 威尔曼光医学中心内，新技术和试点项目将在波士顿建立将进行实验。然而，常规活体显微镜实验将在休斯顿新的 CABIR 大楼内进行，该大楼专门设计了临时设施。高级活体显微镜核心由 Yun 博士领导，并与莱斯大学的 Richards-Kortum 博士和 Lin 博士合作。云博士（物理学博士）是哈佛医学院马萨诸塞州总医院韦尔曼光医学中心的助理教授。核心的活动将与 Wellman 光医学中心的林博士合作开发云博士和林博士研究小组由外部资助的博士后和研究生共约 20 名，在过去 3 年在Nature、Nature Medicine、Nature Photonics、Blood、Journal of Experimental Medicine 以及顶级光学期刊。 Richards-Kortum 博士（物理学博士）是莱斯大学 Stanley C. Moore 教授兼生物工程系系主任。先进的活体显微镜核心将利用其技术工具和专业知识来（i）重建感兴趣区域内的血管结构（直径、长度、弯曲度、渗透性）和流体动力学条件（流速、壁上的剪切应力）； (ii) 可视化纳米和微米尺寸颗粒在脉管系统和肿瘤微环境内的运输，特别测量它们与内皮的粘附率、从血管隔室的外渗率、上皮内的扩散率，正常细胞和肿瘤细胞的内化率和可能的胞吐作用。将通过微创剖腹手术使用微型前视探头分析纳米颗粒的运输。