Development of stimulated Brillouin microscopy for high-resolution stiffness measurement

开发用于高分辨率刚度测量的受激布里渊显微镜

基本信息

批准号：
10592235
负责人：
Seok-Hyun Andy Yun
金额：
$ 45.8万
依托单位：
MASSACHUSETTS GENERAL HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-06-01 至 2025-02-28
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10592235
关键词：
3-Dimensional Adoption Affect Area Atomic Force Microscopy Behavior Biocompatible Materials Biological Biological Process Biomechanics Cells Cellular Morphology Cytoskeleton Detection Development Devices Diagnosis Disease Elements Environment Extracellular Matrix Extracellular Space Fostering Frequencies Goals Hardness Health Hydrogels Image In Situ Interferometry Label Lasers Maps Measurement Measures Mechanics Medical Microscope Microscopic Microscopy Mission Modality Modeling Modulus Movement Nature Neoplasm Metastasis Ophthalmologic Surgical Procedures Optics Organism Organoids Phenotype Physiologic pulse Play Process Proliferating Property Public Health Research Resolution Role Sampling Scheme Science Slice Source Spectrum Analysis Speed Stress Surface System Techniques Technology Time Tissues United States National Institutes of Health Work biomechanical test bioscaffold data acquisition disease diagnosis elastography experience extracellular haptics improved in vivo innovation light scattering mechanical load mechanical properties mechanical signal millimeter millisecond molecular scale novel physical science stem cell differentiation stem cell therapy tissue regeneration tool ultrasound waveguide wound healing

项目摘要

Abstract Cells inside tissues and biomaterials receive mechanical cues that affect the cells’ morphology and function. These phenotype changes in turn can remodel the extracellular environment. To understand this intricate interaction, it is fundamentally necessary to measure the mechanical properties of both intracellular and extracellular space in situ. However, this measurement is very difficult by the lack of suitable tools. The goal of this project is to develop novel optical tools based on stimulated Brillouin scattering (SBS) for measuring and mapping stiffness with 3D resolution. The first specific aim is to develop a longitudinal-wave SBS microscope system using a pulsed scheme to achieve sub-millisecond data acquisition speed, 1000-fold improved over spontaneous Brillouin microscopy. The second specific aim is to develop a shear-wave SBS microscope system. Using the coherent nature of SBS, this new modality may measure shear modulus in a broad range from 100 Pa to 1 MPa with subcellular resolution. The third specific aim is to combine the two systems with a common sample stage allowing both longitudinal and shear moduli to be measured from each microscopic volume. The combined microscope will provide unprecedented label-free, high-resolution access to the full compliance or stiffness tensors of cells, tissues, and hydrogels. The successful development of SBS microscopy will have a major impact on many areas that require mechanical characterization of biomaterials, cells, organoids, tissue slices, and small living organisms at high spatial resolution.

抽象的组织和生物材料内部的细胞会收到影响细胞形态和功能的机械提示。这些表型的变化反过来可以重塑细胞外环境。理解这一复杂的相互作用，从根本上必须测量细胞内和原位细胞外空间。但是，由于缺乏合适的工具，这种测量非常困难。目标这个项目的是开发基于刺激的布里鲁因散射（SB）的新型光学工具并用3D分辨率映射刚度。第一个具体目的是开发纵向波SBS 显微镜系统使用脉冲方案实现亚毫秒的数据采集速度，1000倍改进了赞助的布里渊显微镜。第二个特定目的是开发剪切波SBS 显微镜系统。使用SBS的连贯性质，这种新模式可以测量剪切模量宽范围从100 pA到1 MPa，具有亚细胞分辨率。第三个具体目的是结合两个具有共同样品阶段的系统，可以从每个纵向和剪切模量微观体积。组合显微镜将提供前所未有的无标签，高分辨率访问对细胞，组织和水凝胶的完整合规性或刚度张量。成功的发展 SBS显微镜将对需要机械表征的许多领域产生重大影响生物材料，细胞，器官，组织切片和小型生物体以高空间分辨率。