Laser particles for multi-dimensional single-cell analysis

用于多维单细胞分析的激光粒子

基本信息

批准号：
10495208
负责人：
Seok-Hyun Andy Yun
金额：
$ 74.58万
依托单位：
MASSACHUSETTS GENERAL HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-30 至 2026-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10495208
关键词：
Antibodies Area Award Bar Codes Behavior Biological Sciences Car Phone Cell physiology Cells Cellular Phone Color Communication DNA Data Data Set Development Devices Diagnosis Diagnostic Dimensions Disease Environment Frequencies Goals Healthcare Image In Situ Individual Isotopes Lasers Learning Life Light Location Methodology Molecular Movement Oligonucleotides Optics Persons Physiology Reader Research Series Signal Transduction System Techniques Technology Telephone Therapeutic Time Tissues United States National Institutes of Health Validation Work biological systems biomaterial compatibility cell behavior experimental study fighting fluorophore improved in vivo in vivo imaging innovation insight instrument large scale data nanoparticle new technology next generation novel nucleobase particle single cell analysis single cell sequencing success tool validation studies wireless

项目摘要

Project Summary The broad objective of this proposed work is to advance single-cell analysis by introducing novel cell-barcoding technologies. Understanding cells is a cornerstone of life sciences. Single-cell sequencing led to a paradigm shift in our approach to analyze cells from ensembles to individual cells. Unfortunately, the current single-cell analysis techniques are almost exclusively static, performed ex vivo, and thus cannot easily probe the dynamic cellular processes. Beyond static analysis, the next important step is to add more dimensions: everything that makes a cell a living entity, including temporal changes, spatial movement, interactions with other cells in vivo. This project will develop laser particles (LPs), which can be read optically in real time and repeatedly as needed, for multi-dimensional single-cell analysis. With the optical barcode, it is possible to acquire data from a cell at different times, locations and apparatuses, and compile the data to produce a full account of the cell. A combination of LPs with oligonucleotide barcodes enables a breakthrough approach acquiring large-scale data from in vivo physiology to molecular omics using multiple instruments in series. This project is organized in three specific aims. Aim 1 develops large-scale LP barcodes combined with oligo barcodes. Aim 2 constructs several instruments integrating LP barcode readers into commercial platforms. Aim 3 involves validation studies to demonstrate the immediate utilities of the new technologies. The optical barcoding is expected to make a paradigm change in single-cell analysis. This will transform the way multi-dimensional single-cell analysis is used for scientific discovery and diagnostic and therapeutic applications in healthcare.

项目摘要这项拟议工作的广泛目标是通过引入新型细胞 - 巴尔计数来推进单细胞分析技术。了解细胞是生命科学的基石。单细胞测序导致范式我们分析细胞从共同体到单个细胞的方法转移。不幸的是，当前的单细胞分析技术几乎是静态的，在体内进行，因此无法轻易探测动态细胞过程。除了静态分析之外，下一个重要的步骤是添加更多维度：使一个细胞成为生命实体，包括时间变化，空间运动，与其他细胞的相互作用。该项目将开发激光颗粒（LPS），可以实时进行光学读取，并反复为需要进行多维单细胞分析。使用光条形码，可以从在不同时间，位置和设备的细胞，并编译数据以产生细胞的完整说明。一个 LP与寡核苷酸条形码的组合可以实现一种突破性方法，以获取大规模数据从体内生理学到分子量序列化的分子量。这个项目在三个具体目标。 AIM 1开发大型LP条形码与寡核条形码结合。目标2结构几种将LP条形码读取器集成到商业平台中的乐器。 AIM 3涉及验证研究证明了新技术的直接实用性。预计光条形码将在单细胞分析中改变范式。这将改变多维单细胞的方式分析用于医疗保健中的科学发现以及诊断和治疗应用。