Voltage-sensitive dye imaging setup

电压敏感染料成像装置

• 批准号: 8447278
• 负责人: DIETER JAEGER
• 金额: $ 21.57万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8447278
• 关键词: Address; Amplifiers; Area; Auditory; Auditory area; Autistic Disorder; Basal Ganglia; Biology; Brain; Complex; Computer software; Crabs; Development; Dyes; Equipment; Faculty; Feedback; Funding; Ganglia; Generations; Genetic Techniques; Health; Homeostasis; Image; Imaging Techniques; Invertebrate Ganglia; Invertebrates; Leeches; Lobster; Methods; Microscope; Mus; Neurosciences; Optics; Parkinson Disease; Pattern; Research; Research Personnel; Rodent; Signal Transduction; Surveys; System; Technology; Ultrasonography; United States National Institutes of Health; Universities; Work; expectation; in vivo; innovation; interest; neuroregulation; novel; optogenetics; programs; research study; social; tool; vibration; voltage

DESCRIPTION (provided by applicant): We request funding to establish a voltage-sensitive dye (VSD) imaging setup in the Department of Biology at Emory University. This setup will greatly enhance the research capability of the neuroscience faculty in the Department with active NIH funding (Drs. Jaeger, Calabrese, Liu and Prinz). The P.I. (Dr. Jaeger) has a long track record in the research of basal ganglia and cerebellar networks. To extend this analysis to the level of cortical activity modulation due to basal ganglia and cerebellar feedback loops would be innovative, impactful, and timely. VSD imaging of mouse cortical activity in vivo will be a crucial tool to determine the temporal and spatial effects of basal ganglia and cerebellar activation on cortical activity. Similarly, the research of Dr. Liu is concerned with cortical actiity patterns, albeit the activation following social ultrasound signaling in mice on auditory circuits. VSD imaging would allow this work to progress towards a better understanding of spatial and temporal signal flow beyond primary auditory cortex. For both Dr Jaeger and Dr. Liu this technology would be particularly timely to implement soon, as the development of optogenetic stimulation techniques and genetic voltage-sensitive indicators will allow us to address important questions with an expected high impact in novel findings. The work of Drs. Calabrese and Prinz explores the function of invertebrate pattern generation circuits in the leech heartbeat circuit and crab/lobster stomatogastric ganglion, respectively. However, similarly to the question of spatial dynamics of cortical dynamics, these ganglia consist of a spatial network in the respective ganglia with complex activity patterns that can be fruitfully surveyed by voltage-sensitive dye imaging techniques. The equipment we request will allow us to flexibly conduct experiments on the spatial scale required in rodents in vivo (10x10 mm area of interest) and invertebrate ganglia (1x1 mm area of interest) by mounting a state of the art dual CMOS camera system either on an in vivo imaging setup or on an Olympus BX50 microscope. Both setups will share manipulators and amplifiers for simultaneous electrophysiological recordings. A substantial amount of the basic equipment (Olympus BX50 microscope, vibration isolation table, recording amplifiers) needed for this setup will be contributed by the major investigators, and a dedicated room is made available by the Dept. of Biology. The core item requested is the dual camera CMOS system, along with the necessary optical equipment (beam splitter, software, etc), and auxiliary hardware. The expectation from obtaining this setup is that innovative and timely methods will be available to the established research programs of the major investigators, and allow the development of junior faculty as well. The health impact is also highly relevant, in that the research by the Jaeger lab is directly applied to establishing Parkinson's disease mechanisms, work in the Liu lab is relevant to the study of autism, and work in the Calabrese and Prinz labs demonstrate fundamental mechanisms of neuromodulation and homeostasis that underlie understanding of how pathological brain activity may arise as a maladaptation of normal dynamics.

描述（由申请人提供）：我们请求资金在埃默里大学生物系建立电压敏感染料 (VSD) 成像装置。这一设置将在 NIH 的积极资助下（Jaeger、Calabrese、Liu 和 Prinz 博士）极大地增强该系神经科学教师的研究能力。 P.I. （Jaeger 博士）在基底神经节和小脑网络的研究方面拥有长期的记录。将这种分析扩展到基底神经节和小脑反馈环路引起的皮质活动调节水平将是创新的、有影响力的和及时的。小鼠体内皮质活动的 VSD 成像将成为确定基底神经节和小脑激活对皮质活动的时间和空间影响的重要工具。同样，刘博士的研究关注的是皮质活动模式，尽管是在小鼠听觉回路上的社交超声信号激活之后进行的。 VSD 成像将使这项工作能够更好地理解初级听觉皮层之外的空间和时间信号流。对于 Jaeger 博士和刘博士来说，这项技术的实施非常及时，因为光遗传学刺激技术和遗传电压敏感指标的发展将使我们能够解决重要问题，并有望在新发现中产生巨大影响。博士的工作。 Calabrese 和 Prinz 分别探索了水蛭心跳回路和螃蟹/龙虾口胃神经节中无脊椎动物模式生成回路的功能。然而，与皮质动力学的空间动力学问题类似，这些神经节由各自神经节中的空间网络组成，具有复杂的活动模式，可以通过电压敏感染料成像技术进行有效的调查。我们要求的设备将使我们能够通过安装最先进的双 CMOS 摄像头系统，灵活地在啮齿动物体内（10x10 毫米感兴趣区域）和无脊椎动物神经节（1x1 毫米感兴趣区域）所需的空间尺度上进行实验。体内成像装置或奥林巴斯 BX50 显微镜。两种设置将共享操纵器和放大器，以进行同步电生理记录。该装置所需的大量基本设备（奥林巴斯 BX50 显微镜、隔振台、记录放大器）将由主要研究人员提供，并且生物学系提供了一个专用房间。所要求的核心项目是双摄像头CMOS系统，以及必要的光学设备（分光镜、软件等）和辅助硬件。获得这一设置的期望是，创新和及时的方法将为主要研究者已建立的研究项目提供支持，并允许初级教师的发展。对健康的影响也高度相关，因为 Jaeger 实验室的研究直接应用于建立帕金森病机制，Liu 实验室的工作与自闭症研究相关，Calabrese 和 Prinz 实验室的工作展示了帕金森病的基本机制。神经调节和稳态是理解病理性大脑活动如何作为正常动态适应不良而出现的基础。