Combining bioavailability assays with modeling to predict PCBs in fish after reme

将生物利用度测定与建模相结合来预测修复后鱼类中的 PCB

• 批准号: 8336827
• 负责人: Upal Ghosh
• 金额: $ 27.74万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE COUNTY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-21 至 2014-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8336827
• 关键词: Amendment; Biological Assay; Biological Availability; Carbon; Clams; Decision Making; Development; Devices; Diffusion; Ecosystem; Effectiveness; Equilibrium; Exposure to; Fishes; Fluorescence Microscopy; Food Webs; Health; Human; In Situ; Invertebrates; Kinetics; Laboratories; Lead; Link; Measurement; Measures; Methods; Modeling; Motivation; Nature; Organism; Outcome; Pathway interactions; Pesticides; Poison; Polychlorinated Biphenyls; Process; Research; Research Personnel; Research Project Grants; Risk; Rivers; Sampling; Science; Sectioning technique; Site; Superfund; Techniques; Technology; Testing; Time; Translations; Water; Work; base; bioaccumulation; delrin; improved; interest; mathematical model; remediation; research study; uptake

Title: Combining bioavailability assays with modeling to predict PCBs in fish after remediation Project summary Ecological and human health impacts of bioaccumulative contaminants like PCBs are primarily manifested through accumulation of the toxic compounds in higher trophic level organisms like fish that are consumed by humans and top predators in the ecosystem. However, changes in fish are slow to manifest as a consequence of a remedial action and often one has to wait for several years to see such change. To make timely assessments of remediation progress, one alternative is to perform appropriate measurements that indicate changes in key pathways of exposure to fish. Although some advances have been made recently to assess porewater concentrations using passive sampling techniques which respond more rapidly to in-situ remedies, relationship of such measures to accumulation is fish has not been demonstrated. Also, there is a major gap in the development and utilization of fate and biouptake models that can use passive sampling measurements and quantitatively link those measurements to uptake pathways and predict eventual changes in fish concentrations. This proposed research project will refine sampling methods to assess PCB uptake pathways and work with practitioners to incorporate such measures into PCB fate and biouptake models to assess changes in fish concentration over time, and validate the approach through controlled laboratory exposure studies and measurements in the field. The three primary aims of this project are: Specific aim 1: Develop the fundamental basis of passive sampling. This research will use advanced fluorescence microscopy, IR microspectroscopy, and sectioning techniques to directly measure the diffusion of organic molecules in commonly used passive sampler materials (polyethylene, polyoxymethylene, and PDMS). This effort will lead to the selection and use of appropriate polymeric materials for passive equilibrium sampling with much greater confidence about the nature of equilibrium achieved during the exposure period. Specific aim 2: Use passive sampling to measure bioavailability processes and uptake in fish. This research aim will evaluate how sediments amended with activated carbon sorbents in the field impact PCB biouptake in two types of fish through controlled laboratory mesocosm studies and compare with uptake in passive sampling devices developed under Aim 1. Specific aim 3: Incorporate passive sampling inputs to PCB fate and bioaccumulation model. A mathematical model will be developed and used to interpret results from: 1) the mesocosm exposure experiments, and 2) field observations from a PCB-impacted river site to explore the effect of activated carbon treatments on PCB accumulation in fish. Of particular interest is improving the accuracy of model predictions of the benefits of in-situ treatment with activated carbon aimed at reducing pore water concentrations and contaminant bioavailability.

标题：将生物利用度测定与建模相结合来预测修复后鱼类中的 PCB 项目概要 PCB 等生物累积性污染物对生态和人类健康的影响主要是 通过有毒化合物在较高营养级生物体（如鱼类）中的积累而表现出来 被生态系统中的人类和顶级掠食者消耗。然而，鱼类的变化却是缓慢的。 作为补救措施的结果而显现出来，通常需要等待数年才能看到这种情况 改变。为了及时评估补救进度，一种替代方法是执行适当的补救措施 表明接触鱼类的主要途径发生变化的测量结果。尽管取得了一些进展 最近使用被动采样技术评估孔隙水浓度，该技术响应 就地补救措施而言，这种措施与鱼类积累之间的关系尚未得到证实。 证明了。此外，命运和生物摄取模型的开发和利用还存在重大差距 可以使用被动采样测量并将这些测量定量地与吸收联系起来 路径并预测鱼类浓度的最终变化。该拟议研究项目将完善 评估 PCB 吸收途径的采样方法，并与从业人员合作将此类方法纳入其中 测量 PCB 归宿和生物吸收模型，以评估鱼类浓度随时间的变化，以及 通过受控实验室暴露研究和现场测量来验证该方法。这 该项目的三个主要目标是： 具体目标 1：开发被动采样的基本基础。这项研究将使用先进的 荧光显微镜、红外显微光谱和切片技术可直接测量 有机分子在常用被动采样器材料（聚乙烯、 聚甲醛和 PDMS）。这项努力将导致选择和使用适当的聚合物 用于被动平衡采样的材料，对平衡的性质有更大的信心 暴露期间取得的成果。 具体目标 2：使用被动采样来测量鱼类的生物利用度过程和吸收。这 研究目的将评估活性炭吸附剂在现场修正沉积物的影响 通过受控实验室中生态系统研究，两种鱼类对 PCB 的生物吸收并与 目标 1 下开发的被动采样装置的采用。 具体目标 3：将被动采样输入纳入 PCB 归宿和生物累积模型。一个 将开发数学模型并用于解释以下结果：1）中宇宙暴露 实验，以及 2) 对受 PCB 影响的河流地点进行现场观察，以探索活化的影响 碳处理对鱼类 PCB 积累的影响。特别令人感兴趣的是提高模型的准确性 预测活性炭原位处理旨在减少孔隙水的好处 浓度和污染物的生物利用度。