Diffuse Intrinsic Pontine Glioma Genomic Study

弥漫性内在脑桥胶质瘤基因组研究

• 批准号: 8763309
• 负责人: Katherine e Warren
• 金额: $ 5.14万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8763309
• 关键词: 10 year old; Age; Area; Autopsy; Biopsy; Brain Stem Glioma; Cessation of life; Child; Consensus; DNA; Data; Diffuse; Employee Strikes; Excision; Freezing; Genes; Genetic; Genomics; Glioma; Immunohistochemistry; Institution; Life; Location; Manuscripts; Mutation; Operative Surgical Procedures; Paraffin Embedding; Pathology; Pathway interactions; Patients; Pontine structure; Procedures; Process; Publishing; Resolution; Risk; Sampling; Societies; Specimen; Techniques; Time; Tissues; Tumor Biology; comparative genomic hybridization; interest; meetings; neuro-oncology; outcome forecast; tumor; tumor progression

Twenty-eight paraffin-embedded tissue blocks and fresh frozen samples obtained from autopsy specimens have been collected from patients treated at NCI and outside institutions. These are currently undergoing pathology confirmation and immunohistochemistry studies. A representative area of tumor is collected from each sample and DNA extracted from the core with standard procedures. Tumor DNA is processed for high resolution comparative genomic hybridization. Regions of copy number change have been identified using circular binary segmentation of the array data. Consensus regions across specimens will be identified and interrogated for genes and pathways related to tumor progression. Results of this study were presented at the Society of Neuro-Oncology meeting 2009. The study will be expanded to include all grades of gliomas. A manuscript is been published in Neuro-Oncology.

从NCI和外部机构治疗的患者中收集了从尸检标本中获得的28个石蜡包裹的组织块和新鲜的冷冻样品。这些目前正在接受病理学确认和免疫组织化学研究。从每个样品中收集了肿瘤的代表性区域，并通过标准程序从核心提取DNA。处理肿瘤DNA以进行高分辨率比较基因组杂交。使用阵列数据的圆形二进制分段已经确定了拷贝数更改的区域。对于与肿瘤进展相关的基因和途径，将识别和审问跨标本的共识区域。这项研究的结果在2009年神经肿瘤学会会议上介绍。该研究将扩展到包括所有等级的神经胶质瘤。手稿在神经肿瘤学上发表。