ICM Core

ICM核心

• 批准号: 8517264
• 负责人: ADRIAN OZINSKY
• 金额: $ 18.32万
• 依托单位: INSTITUTE FOR SYSTEMS BIOLOGY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8517264
• 关键词: Applications Grants; Automation; Bar Codes; Binding Sites; Biochemical; Biological Assay; Biology; Carbon; Carrying Capacities; Cell Culture Techniques; Cell Wall; Cell surface; Cells; Characteristics; Collaborations; Complex; Concanavalin A; Convection; Cytolysis; Cytometry; Data; Development; Devices; Dissection; Drug Interactions; Educational process of instructing; Elements; Ensure; Environment; Equipment; Figs - dietary; Fostering; Funding; Future; Gene Mutation; Generations; Genetic; Genome; Genus staphylococcus; Glucose; Glycopeptides; Goals; Growth; Image; Image Analysis; Image Cytometry; Incubators; Individual; Label; Lasers; Legal patent; Life; Liquid substance; Manuals; Measurement; Measures; Messenger RNA; Methods; MicroRNAs; Microfluidics; Microscope; Microscopy; Modeling; Modification; Monitor; Nucleic Acids; Oleic Acids; Organism; Pharmaceutical Preparations; Phenotype; Physiologic pulse; Polymers; Population; Population Heterogeneity; Positioning Attribute; Procedures; Process; Proteins; Protocols documentation; Publications; Quantum Dots; RNA; Regimen; Request for Proposals; Research; Research Personnel; Resolution; Resources; Running; Scanning; Slide; Solid; Source; Staging; Surface; System; Systems Biology; Techniques; Technology; Time; Training; Training and Education; Vacuum; Yeasts; base; cell growth; cellular imaging; computerized tools; design; dosage; instrument; interest; lithography; microorganism; mutant; next generation; operation; photonics; research study; response; single cell analysis; skills; success; technology development; tool; two-dimensional; web site

Imaging Cytometry and Microfluidics (ICM) core I (Ozinsky) Overview. The core was established by the Center to provide the resources for automated high-throughput imaging experiments on live cells along with cell purification and analysis. We have contributed to research resulting in 13 publications and two patent applications involving the analysis of cells across bacterial, yeast and mammalian systems. This has included projects for the purification and analysis of single cells, large-scale imaging screens, analysis of subcellular dynamics, and the development of new methods devices and computational tools. The core is staffed by a director, full-time manager,and a full-time technician, and has open access to state-of-the-art microscopy, cytometry, and microfluidics (see Resources). The core provides comprehensive training for users to operate equipment independently, and maintains a website with manuals and protocols to ensure standardized procedures. The ICM core has integrated control of microscopes, fluidics, image acquisition and analysis under Lab-VIEW (National Instruments) and MatLab (MathWorks). Three cell culture incubators are available to enable time-lapse/live-cell imaging on these workstations. Our dedicated clean room has a variety of soft lithography equipment including: a PDMS pre polymer mixer, a spin-coater, a dissection microscope for PDMS device layer alignment, a vacuum dessicator, PDMS punches, and a dedicated 80¿C convection oven. Further, we have established an annual three-day Microfluidics course to teach the fundamental skills for PDMS design, fabrication, and operation (see Education and Training Plan).

成像细胞术和微流控 (ICM) 核心 I (Ozinsky) 概述 该核心由该中心建立，旨在为活细胞的自动化高通量成像实验以及细胞纯化和分析提供资源，我们已发表了 13 篇出版物和两项涉及细菌细胞分析的专利申请。 、酵母和哺乳动物系统，这包括单细胞的纯化和分析、大规模成像动力学屏幕、亚细胞分析以及新方法设备和计算工具的开发项目，其核心人员由一名主任组成。 -时间经理，以及全职技术人员，可以使用最先进的显微镜、细胞术和微流体技术 （参见参考资料）。核心为用户提供独立操作设备的全面培训，并维护一个包含手册和协议的网站以确保标准化的程序。 ICM 核心在 Lab-VIEW (National Instruments) 和 MatLab (MathWorks) 下集成了显微镜、流体、图像采集和分析控制，可在这些工作站上实现延时/活细胞成像。专用洁净室拥有各种软光刻设备，包括：PDMS预聚物混合器、旋涂机、用于PDMS器件层对准的解剖显微镜、真空干燥器、PDMS冲头和专用80°此外，我们还设立了每年为期三天的微流体课程，教授 PDMS 设计、制造和操作的基本技能（请参阅教育和培训计划）。