PROTEOMIC PROFILING OF INTEGRAL MEMBRANE PROTEIN TOPOLOGY

整体膜蛋白拓扑结构的蛋白质组学分析

• 批准号: 8365856
• 负责人: Christine Wu Nordahl
• 金额: $ 0.65万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365856
• 关键词: Biochemical; Biology; Digestion; Funding; Fungal Genome; Goals; Grant; Integral Membrane Protein; Lipid Bilayers; Membrane Proteins; National Center for Research Resources; Peptides; Principal Investigator; Proteomics; Research; Research Infrastructure; Resources; Source; United States National Institutes of Health; cost; improved

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of the project is to use proteolytic digestion combined with the characterization of the resulting peptides to improve our understanding of membrane protein topology. Biochemical approaches are being applied to characterize both the soluble "loops" and the lipid bilayer embedded peptides.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 该项目的目标是利用蛋白水解消化结合所得肽的表征来提高我们对膜蛋白拓扑结构的理解。生化方法被用来表征可溶性“环”和脂质双层嵌入的肽。