COMPARATIVE PROTEOMICS AND GLYCOPROTEOMICS OF PROTEINS SECRETED FROM B CINEREA

灰霉病菌分泌蛋白质的比较蛋白质组学和糖蛋白质组学

• 批准号: 8363013
• 负责人: RON ORLANDO
• 金额: $ 6.02万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363013
• 关键词: Affect; Botrytis; Cell Wall; Development; Economics; Enzymes; Flowers; Funding; Grant; Gray unit of radiation dose; Growth; Harvest; Infection; Molds; National Center for Research Resources; Phase; Plants; Prevention strategy; Principal Investigator; Process; Proteins; Proteome; Proteomics; Recombinant Proteins; Research; Research Infrastructure; Resources; Source; Technology; Tissues; Trees; United States National Institutes of Health; comparative; cost; fruits and vegetables; fungus; glycosylation; pathogen; protein structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The fungus Botrytis cinerea is a widespread plant pathogen that affects many vegetables and fruits, as well as a large number of shrubs, trees, flowers, and weeds by causing grey mold or soft rot on the host tissues. B. cinerea mainly infects dicotyledons and non-graminaceous monocotyledons, and the infection not only occurs during the growth phase of crops but also during storage of the harvested products. Thus, it is responsible for significant economic losses. A great deal of research has focused on understanding the B. cinerea infection process, which in turn directs the development of effective prevention strategies. B. cinerea must breach the cell wall during the infection process and accordingly secretes a great number of enzymes that can degrade cell walls. Little is known concerning the identity of these enzymes. The majority of these cell wall degrading enzymes are glycosylated. Although the glycosylation on several recombinant proteins have been characterized, very little is known on the native enzymes, despite the importance of glycosylation on protein structure and activity. To this end, we have started to characterize the secreted proteome and glycoproteome of this fungus.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 真菌辣椒粉是一种广泛的植物病原体，会影响许多蔬菜和水果，以及大量灌木，树木，花朵和杂草，通过在宿主组织上引起灰色霉菌或软腐烂。 B.灰果杆菌主要感染双子叶植物和非同性恋单子叶植物，并且感染不仅发生在农作物的生长阶段，而且还发生在收获产物的储存过程中。因此，它是造成重大经济损失的原因。大量的研究集中在理解B. cinerea感染过程上，这反过来又指导了有效的预防策略的发展。 B.灰果会在感染过程中破坏细胞壁，因此分泌大量会降解细胞壁的酶。关于这些酶的身份知之甚少。这些细胞壁降解酶的大多数是糖基化的。尽管已经表征了几种重组蛋白上的糖基化，但尽管糖基化对蛋白质结构和活性的重要性，但在天然酶上几乎没有知名度。为此，我们已经开始表征这种真菌的分泌蛋白质组和糖蛋白酶。