GLYCOPROTEIN GLYCOMICS: EXPRESSION IN MOUSE ES CELLS & PROGENY

糖蛋白糖组学：小鼠 ES 细胞中的表达

• 批准号: 8363005
• 负责人: RON ORLANDO
• 金额: $ 6.02万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363005
• 关键词: Affinity Chromatography; Bioinformatics; Cataloging; Catalogs; Cells; Data; Embryo; Fractionation; Funding; Glycopeptides; Glycoproteins; Grant; Lectin; Link; Liquid Chromatography; Mass Spectrum Analysis; Mus; National Center for Research Resources; Peptide N-Glycosidase; Peptides; Polysaccharides; Principal Investigator; Research; Research Infrastructure; Resources; Source; Staging; Stem cells; Technology; United States National Institutes of Health; cost; embryonic stem cell; liquid chromatography mass spectrometry; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Core 2A is developing tools to analyze the Glycoprotein Glycome of cells, including embryonic mouse stem cells and their differentiated products. Fractionation of tryptic glycopeptides using serial lectin affinity chromatography is being developed to classify expression by the types of glycans expressed. Separation of glycopeptides is then further accomplished by liquid chromatography, followed by mass spectrometry to catalog and identify various species. At various stages, N-linked glycans will be produced by N-glycanase treatment. The resulting peptides will be identified by their Asn to Asp conversion using LC/MS analysis. All results will be submitted to the Bioinformatics module (Core project 4) for integration with other data.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 Core 2a正在开发工具来分析细胞的糖蛋白糖果，包括胚胎小鼠干细胞及其分化产物。正在开发使用串行凝集素亲和色谱法分解胰蛋白酶糖肽，以通过表达的聚糖类型对表达进行分类。然后，通过液相色谱进一步进一步完成糖肽的分离，然后进行质谱法以目录并鉴定各种物种。在各个阶段，N连接的聚糖将通过N-糖果酶处理产生。通过LC/MS分析，将通过其ASN转换为ASN鉴定所得的肽。所有结果将提交给生物信息学模块（核心项目4），以与其他数据集成。