DEVELOPMENT OF TECHNIQUES FOR QUANTITATIVE GLYCOMICS

定量糖组学技术的发展

• 批准号: 8363021
• 负责人: RON ORLANDO
• 金额: $ 3.44万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363021
• 关键词: Affinity; Cations; Complex Mixtures; Funding; Grant; Individual; Mass Spectrum Analysis; Methodology; Methods; Molecular; National Center for Research Resources; Polysaccharides; Principal Investigator; Protons; Research; Research Infrastructure; Resources; Source; Structure; Surface; Technology; United States National Institutes of Health; comparative; cost; improved; ionization; molecular mass; research study; technique development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The quantitative comparison of glycan expression requires highly sensitive methods that can distinguish and identify individual glycans with subtly different structures. Mass spectrometry (MS) fulfills these requirements while providing a rapid and reliable method to analyze complex mixtures, and therefore has been widely used in glycomic studies. One limitation of using MS for the quantitation of biomolecules is that the ionization efficiencies of distinct molecular species can differ significantly, depending on such factors as the analyte's molecular mass, proton/cation affinity, surface activity, the presence of compounds which compete with or interfere with the ionization of the analyte, etc. We are involved in devising new methodologies to improve the accuracy of comparative glycomic experiments.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 聚糖表达的定量比较需要高度敏感的方法，该方法可以区分和鉴定具有微妙结构的单个聚糖。 质谱（MS）满足了这些要求，同时提供了一种快速可靠的方法来分析复杂混合物，因此已广泛用于血糖研究中。 使用MS定量生物分子的一种局限性是，不同分子物种的电离效率可能会有很大差异，这取决于分析物的分子质量，质子/阳离子亲和力，表面活性，与或竞争或干扰或干扰的化合物的存在。随着分析物等的电离。我们参与了设计新方法以提高比较糖化实验的准确性。