TECHNOLOGY DEVELOPMENT FOR ISOLATING GPI-ANCHORED GLYCOPROTEINS

分离 GPI 锚定糖蛋白的技术开发

• 批准号: 8363123
• 负责人: J. Michael Pierce
• 金额: $ 3.44万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363123
• 关键词: Binding; Biological Markers; Cells; Clostridium; Disease; Funding; Glycoproteins; Goals; Grant; Malignant Neoplasms; Mining; National Center for Research Resources; Principal Investigator; Proteins; Reagent; Research; Research Infrastructure; Resources; Serum; Source; Techniques; Technology; Tissues; United States National Institutes of Health; Work; alpha Toxin; cost; technology development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This subproject's goal is to develop a technique to identify the class of glycoproteins in cells known as glycosylphosphoinositide-anchored proteins. This class of proteins is known to increase in size during some disease states, such as cancer, both in cancer tissues and in serum, and it is a logical class to mine for potential biomarkers. The reagent we have been working with is the alpha-toxin from Clostridium septicans, which is known to bind some GPI-anchored proteins.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 该亚参数的目标是开发一种技术，以鉴定称为糖基磷酸肌醇锚定蛋白的细胞中的糖蛋白类别。 众所周知，这种蛋白质在某些疾病状态（例如癌症组织和血清）中的大小会增加，这是一个可以挖掘潜在生物标志物的逻辑类别。 我们一直在使用的试剂是盐梭状芽胞杆菌的α-毒素，该试剂已知可以结合一些GPI锚定蛋白。