MOUSE BRAIN GLYCOPROTEINS EXPRESSING O-MAN AND ASN-LINKED GLYCANS

表达 O-MAN 和 ASN 连接聚糖的小鼠脑糖蛋白

• 批准号: 8363012
• 负责人: J. Michael Pierce
• 金额: $ 0.34万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363012
• 关键词: Brain; Cell-Cell Adhesion; Enzymes; Funding; Glycoproteins; Grant; Knockout Mice; Link; Mannose; Mass Spectrum Analysis; Mus; National Center for Research Resources; Neurons; Polysaccharides; Principal Investigator; Research; Research Infrastructure; Resources; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Techniques; Technology; United States National Institutes of Health; brain tissue; cell motility; cost; glycosyltransferase; man; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A relatively new type of O-linked glycan has been implicated in neuronal cell adhesion and migration, the O-linked mannose glycans. This project will use novel techniques to separate glycoproteins expressing O-Man glycans from brain tissue and identify these glycoproteins using mass spectrometry techniques. In addition, the Asn-linked glycans from mouse brain have been identified by MALDI-TOF MS. These are being compared to glycans found in mice that are null for the GnT-Va glycosyltransferase to determine how this enzyme regulates glycoprotein glycan expression. A GnT-Vb null mouse has now been produced and the effects of this deletion on O-Man and Asn-linked glycoproteins is now under study.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 一种相对较新的O连锁聚糖与神经元细胞的粘附和迁移有关，即O连接的甘露糖糖。 该项目将使用新颖的技术来将表达O-Man聚糖从脑组织中表达O-MAN的糖蛋白分离，并使用质谱技术鉴定这些糖蛋白。此外，已经通过MALDI-TOF MS鉴定出来自小鼠脑的ASN连接聚糖。将这些与GNT-VA糖基转移酶无效的小鼠中发现的聚糖进行了比较，以确定该酶如何调节糖蛋白聚糖的表达。 现在已经产生了一种GNT-VB无效小鼠，并且该缺失对O-MAN和ASN连接糖蛋白的影响现在正在研究中。