SIMULATION NEUROTRANSMITTER DIFFUSION IN CEREBELLAR GLOMERULI

模拟小脑肾小球中的神经递质扩散

• 批准号: 7956214
• 负责人: TERRENCE J SEJNOWSKI
• 金额: $ 0.09万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7956214
• 关键词: Address; Biomedical Research; Chemicals; Computer Retrieval of Information on Scientific Projects Database; Diffusion; Electrons; Extracellular Space; Funding; Grant; High Performance Computing; Institution; Modeling; Neurotransmitters; Physiology; Request for Proposals; Research; Research Personnel; Resources; Services; Simulate; Site; Source; Structure; Synapses; United States National Institutes of Health; base; chemical release; neurotransmission; reconstruction; simulation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The aim of this project is to examine the influence of the geometry of extracellular space on diffusion of neurotransmitter within specialized synaptic structures called cerebellar glomeruli. We focus on the cerebellar glomeruli since their unique physiology allows a non-classical type of chemical neurotransmission whereby chemicals released at a given synaptic contact are able to reach nearby synaptic contacts. This allows us to ask whether release sites within a glomerulus are arranged with respect to each other and with respect to various structural features in such a way as to yield a different effective diffusion coefficient than one obtained with a random distribution of release sites. In other words are diffusion paths between actual release sites uniquely set up to either facilitate or hamper diffusion of neurotransmitter between them? To address this question we will simulate spillover diffusion in models of cerebellar glomeruli whose geometry is based on electron tomographic reconstructions. This proposal is the second of two part proposal setup. The 1st portion was sent out in the middle of September 2007, as a DAC proposal for 30000 service units. This proposal requests the remaining 114000 service units.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 该项目的目的是检查细胞外空间的几何形状对神经递质在称为小脑肾小球的特殊突触结构内扩散的影响。我们关注小脑肾小球，因为它们独特的生理学允许非经典类型的化学神经传递，从而在给定突触接触处释放的化学物质能够到达附近的突触接触。这使我们能够询问肾小球内的释放位点是否相对于彼此以及相对于各种结构特征排列，从而产生与释放位点随机分布所获得的有效扩散系数不同的有效扩散系数。换句话说，实际释放位点之间的扩散路径是否被独特地设置以促进或阻碍神经递质在它们之间的扩散？为了解决这个问题，我们将在小脑肾小球模型中模拟溢出扩散，其几何形状基于电子断层扫描重建。该提案是两部分提案设置中的第二部分。第一部分于 2007 年 9 月中旬发出，作为 30000 个服务单元的 DAC 提案。该提案要求剩余的114000个服务单位。