OXIDATIVE POST-TRANSLATIONAL MODIFICATIONS IN CARDIOVASCULAR DISEASE

心血管疾病中的氧化翻译后修饰

• 批准号: 8365547
• 负责人: Catherine E. Costello
• 金额: $ 2万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-08-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365547
• 关键词: Biological; Biology; Cardiovascular Diseases; Clinical Investigator; Computer software; Foundations; Fourier Transform; Funding; Gel; Grant; High Pressure Liquid Chromatography; Laboratories; Mass Spectrum Analysis; Medicine; Methods; National Center for Research Resources; National Heart, Lung, and Blood Institute; Oxidative Stress; Paper; Peptides; Performance; Post-Translational Protein Processing; Principal Investigator; Proteins; Proteolysis; Proteomics; Reporting; Research; Research Infrastructure; Resources; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; United States National Institutes of Health; Whole Organism; computerized data processing; cost; cryogenics; mass spectrometer; meetings; novel; tissue/cell culture

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In the first 7-year period of its funding, the research of the NHLBI-sponsored Cardiovasculaar Proteomics Center focused on exploring the oxidative post-translational modifications that occur in conjunction with cardiovascular disease. The Core laboratory developed appropriate methods and provided capLC-QTOF analysis of peptides in digests of 2D-gel separated or multistage HPLC purified proteins. In conjunction with the Resource, MALDI-TOF MS, LTQ-Orbitrap MS/MS and FTMS mass spectral analyses were also carried out. Software to assist in data processing and interpretation was generated in the Center. A novel VC-MALDI cryogenic Fourier transform mass spectrometer with a 14-T magnet was constructed to enable high throughput, high performance analysis of proteolysis mixtures from biological sources, and efficient, accurate determination of their post-translational modifications. Basic and clinical investigators, primarily located at BUSM and HMS, are using proteomic approaches to investigate the effects of oxidative stress on cell cultures, tissues and whole organisms. The first period of the CPC funding extended into the beginning of this reporting year, and a number of the final papers and meeting presentations also were completed. This project laid the foundation for the new project that began in August 2010, and for many proteomics studies that have been undertaken by the MS Resource.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的首席研究员可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 在资助的前 7 年期间，NHLBI 资助的心血管蛋白质组学中心的研究重点是探索与心血管疾病相关的氧化翻译后修饰。核心实验室开发了适当的方法，并对 2D 凝胶分离或多级 HPLC 纯化蛋白质的消化物中的肽进行 capLC-QTOF 分析。结合该资源，还进行了 MALDI-TOF MS、LTQ-Orbitrap MS/MS 和 FTMS 质谱分析。该中心生成了协助数据处理和解释的软件。构建了一种带有 14-T 磁体的新型 VC-MALDI 低温傅里叶变换质谱仪，能够对生物来源的蛋白水解混合物进行高通量、高性能分析，并高效、准确地测定其翻译后修饰。主要位于 BUSM 和 HMS 的基础和临床研究人员正在使用蛋白质组学方法来研究氧化应激对细胞培养物、组织和整个生物体的影响。第一期CPC资助延续至本报告年初，一些最终论文和会议报告也已完成。该项目为 2010 年 8 月开始的新项目以及 MS Resource 开展的许多蛋白质组学研究奠定了基础。