Legacy Support During Closure of the Mass Spectrometry Resource for Biology and Medicine

生物学和医学质谱资源关闭期间的遗留支持

• 批准号: 9810729
• 负责人: Catherine E. Costello
• 金额: $ 82.73万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9810729
• 关键词: Address; Area; Automobile Driving; Bacteria; Biology; Biopolymers; Boston; Cardiovascular Diseases; Cells; Clinical; Communities; Complex; Complex Mixtures; Computer software; Data; Databases; Deposition; Development; Disease; Dissociation; Electrons; Evaluation; Extracellular Matrix; Fostering; Funding; Funding Agency; Future; Glycobiology; Glycolipids; Glycosaminoglycans; Grant; Health; Human; Human Milk; Immunology; Individual; Industry; Industry Collaboration; Infection; Institution; Link; Lipopolysaccharides; Mass Spectrum Analysis; Medicine; Methods; Mission; Monoclonal Antibody R24; National Institute of General Medical Sciences; Parasites; Pathway interactions; Peptides; Policies; Polysaccharides; Postdoctoral Fellow; Proteins; Protocols documentation; Quality Control; Request for Proposals; Research; Resources; Scientist; Structure; Testing; Time; Training; Universities; Variant; base; bioinformatics tool; design; experience; ion mobility; mass spectrometer; protein misfolding; protein protein interaction; recruit; software development; student training; tandem mass spectrometry; technology development; user-friendly

The mission of the Boston University Mass Spectrometry Resource for Biology and Medicine is the development and application of advanced, mass spectrometry-based methods for the characterization of biopolymers that are relevant to human health and disease. Its steady progress has been fostered by close working relationships between basic scientists, clinicians and trainees. Because NIGMS has now imposed a time limit for P41 grants, the grant P41 GM104603 cannot be renewed. This proposal requests the funding essential to avoid a disruptive sudden closedown of the Resource's activities and afford a smooth transition period to enable the orderly completion of ongoing driving biomedical and collaborative projects that depend on methods and expertise uniquely available in the Resource, finalizing of ongoing software development, sharing of software, databases and protocols to meet the needs of the community, and the identification and implementation of alternative mechanisms to address the future needs of the user community and enable further technology developments that should evolve from the accomplishments of the Resource over the last 20 years. The Resource will focus on the needs of glycobiology for detailed structural elucidations and the profiling of complex mixtures of glycans. Reference data that illuminates glycan fragmentation pathways using multiple dissociation methods will be compiled and deposited into public databases; the new, user-friendly bioinformatics tools that the Resource is creating for glycan analysis will be completed, tested and shared. Protocols and sets of reference data for Ion mobility-tandem mass spectrometry will be compiled for the analysis of glycans and complex peptide mixtures, for characterization of noncovalent complexes, and for evaluation of new ExD cell designs being constructed by a small industry collaborator. In-house in developed software for glycan structural determinations and top-down analysis of variant and post-translationally modified proteins, that can establish relationships among PTMs on individual proteins, will be completed and shared; it should provide means for straightforward clinical analyses, and for probing protein-glycan and protein-protein interactions. Translational projects to be completed will include characterization of N- and O-linked glycans and lipopolysaccharides on infectious bacteria and parasites, structural determinations of glycolipids and glycosaminoglycans in human milk, protein misfolding disorders, immunology, extracellular matrix biology and cardiovascular disease. The Resource will transfer its experience in training students, postdoctoral fellows and practicing scientists to other educational institutions and industry.

波士顿大学生物学和医学质谱资源的使命是 先进的、基于质谱的方法的开发和应用 与人类健康和疾病相关的生物聚合物的表征。其稳定 基础科学家、临床医生之间的密切工作关系促进了进展 和实习生。由于 NIGMS 现在对 P41 补助金施加了时间限制，因此 P41 补助金 GM104603 无法续订。该提案要求提供必要的资金，以避免 资源活动的破坏性突然关闭并提供平稳的过渡期 使正在进行的生物医学和合作项目有序完成 取决于资源中独特可用的方法和专业知识，最终确定正在进行的 软件开发、软件、数据库和协议的共享，以满足 社区，以及确定和实施替代机制来解决 用户社区的未来需求，并实现进一步的技术发展 该资源的发展源自过去 20 年的成就。该资源将 重点关注糖生物学对详细结构阐明和分析的需求 聚糖的复杂混合物。阐明聚糖断裂途径的参考数据 使用多种解离方法将被编译并存入公共数据库；这 该资源为聚糖分析创建的新的、用户友好的生物信息学工具将是 已完成、测试并共享。离子淌度串联的协议和参考数据集 质谱法将用于分析聚糖和复杂的肽混合物， 用于非共价复合物的表征，以及用于评估新的 ExD 细胞设计 由小型行业合作者建造。内部开发的聚糖软件 变体和翻译后修饰的结构测定和自上而下分析 能够在单个蛋白质上建立 PTM 之间关系的蛋白质将被完成 并分享；它应该提供直接的临床分析和探索的手段 蛋白质-聚糖和蛋白质-蛋白质相互作用。待完成的翻译项目将 包括 N- 和 O- 连接聚糖和脂多糖对感染性的表征 细菌和寄生虫，糖脂和糖胺聚糖的结构测定 母乳、蛋白质错误折叠疾病、免疫学、细胞外基质生物学和 心血管疾病。该资源将传授其培训学生的经验， 其他教育机构和行业的博士后研究员和执业科学家。