STRUCTURAL BIOLOGY OF SELECTED TARGETS FROM MYCOBACTERIUM TUBERCULOSIS

结核分枝杆菌选定靶标的结构生物学

• 批准号: 8362299
• 负责人: Katherine S Bateman
• 金额: $ 0.16万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362299
• 关键词: Amino Acids; Binding; Data Collection; Drug Metabolic Detoxication; Funding; Gene Targeting; Goals; Grant; Mycobacterium tuberculosis; National Center for Research Resources; Pathway interactions; Phase; Principal Investigator; Proteins; Radiation; Research; Research Infrastructure; Resolution; Resources; Screening procedure; Source; Structure; United States National Institutes of Health; Work; cost; design; expression cloning; inhibitor/antagonist; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We have selected more than 400 target genes from Mycobacterium tuberculosis for cloning, expression and crystal structure determination. The targets are either gene products with an unknown function or are enzymatic activities from several essential pathways involved in detoxification or amino acid synthesis. Over 25 crystal structures have been determined in our lab to date and a number of targets have been cloned, expressed and crystallized. Structural work is continuing on the previously determined crystal structures with the goal of elucidating the detailed enzymatic mechanism for the design of effective inhibitors. The project requires the screening of typically large numbers of crystals, MAD or SAD phasing and high-resolution data collection to visualize the details of substrate or inhibitor binding.