STRUCTURAL BIOLOGY OF SELECTED TARGETS FROM M TUBERCULOSIS FOR THE DESIGN OF NO

用于设计 NO 的结核分枝杆菌选定靶标的结构生物学

• 批准号: 8362087
• 负责人: Katherine S Bateman
• 金额: $ 0.03万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362087
• 关键词: Amino Acids; Binding; Data Collection; Drug Metabolic Detoxication; Funding; Gene Targeting; Goals; Grant; Mycobacterium tuberculosis; National Center for Research Resources; Pathway interactions; Phase; Principal Investigator; Proteins; Radiation; Research; Research Infrastructure; Resolution; Resources; Screening procedure; Source; Structure; United States National Institutes of Health; Work; cost; design; expression cloning; inhibitor/antagonist; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We have selected almost 400 target genes from Mycobacterium tuberculosis for cloning, expression and crystal structure determination. The targets are either gene products with an unknown function or are enzymatic activities from several essential pathways involved in detoxification or amino acid synthesis. 15 crystal structures have been determined to date and a number of targets haven been cloned, expressed and crystallized. Structural work is continuing on the previously determined crystal structures with the goal of elucidating the detailed enzymatic mechanism for the design of effective inhibitors. The project requires the screening of typically large numbers of crystals, MAD or SAD phasing and high-resolution data collection to visualize the details of substrate or inhibitor binding.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 我们从结核分枝杆菌中选择了近400个靶基因来克隆，表达和晶体结构的测定。靶标的是具有未知功能的基因产物，或者是来自涉及排毒或氨基酸合成的几种基本途径的酶活性。已经确定了15个晶体结构，并且尚未克隆，表达和结晶的许多目标。结构性工作正在继续对先前确定的晶体结构进行，目的是阐明设计有效抑制剂的详细酶机制。该项目需要筛选典型的大量晶体，疯狂或悲伤的相位和高分辨率数据收集，以可视化底物或抑制剂结合的细节。