STRUCTURAL STUDIES OF THE EUKARYOTIC TRANSCRIPTION MACHINERY

真核转录机器的结构研究

• 批准号: 8362149
• 负责人: David A. Bushnell
• 金额: $ 1.56万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362149
• 关键词: Complex; DNA Polymerase II; Development; Elongation Factor; Enzymes; Eukaryota; Funding; Gene Expression; Genetic Transcription; Grant; Mediator of activation protein; Messenger RNA; Mutation Analysis; National Center for Research Resources; Pathway interactions; Peptide Initiation Factors; Principal Investigator; Process; Proteins; RNA Polymerase II; Radiation; Regulation; Research; Research Infrastructure; Resources; Source; Transcriptional Regulation; United States National Institutes of Health; cost; polypeptide; structural biology; tumorigenesis

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Transcription is the first step and the key control point in the pathway of gene expression. Transcriptional regulation underlies development, oncogenesis, and other fundamental processes. In eukaryotes the enzyme RNA Polymerase II (pol) is responsible for transcription of messenger RNA making pol?s regulation central to gene expression. During the period of this proposal we will continue our structural studies of the pol transcription machinery. These studies are currently proceeding along three avenues: first, detailed analysis of the elongating pol II using mutation analysis; second, co-crystals of pol II with initiation and elongation factors; third, structural studies of the mediator complex, a 22 polypeptide complex required for pol to respond to regulating proteins.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 转录是基因表达途径中的第一步和关键控制点。转录调节是发展，​​肿瘤发生和其他基本过程的基础。在真核生物中，酶RNA聚合酶II（POL）负责使Messenger RNA的转录使POL的调节与基因表达中心。在此提案期间，我们将继续对POL转录机械进行结构研究。这些研究目前正在沿着三种途径进行：首先使用突变分析对延长的Pol II进行详细分析；其次，Pol II的共结晶与起始和伸长因子；第三，介体复合物的结构研究，这是对调节蛋白质反应的22个多肽复合物。