THE STRUCTURE OF RNA POLYMERASE II AND ASSOCIATED FACTORS

RNA聚合酶II的结构和相关因素

• 批准号: 7721737
• 负责人: David A. Bushnell
• 金额: $ 0.52万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721737
• 关键词: Complex; Computer Retrieval of Information on Scientific Projects Database; DNA; DNA Polymerase II; Enzyme Gene; Eukaryota; Eukaryotic Cell; Funding; Gene Expression; Genetic Transcription; Grant; Institution; Investigation; Messenger RNA; Polymerase; RNA; RNA Polymerase II; Regulation; Research; Research Personnel; Resolution; Resources; Saccharomyces cerevisiae; Site-Directed Mutagenesis; Source; Structure; Transcriptional Regulation; United States National Institutes of Health; Ursidae Family; cell growth; polymerization

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. RNA polymerase II (pol II), the central enzyme of gene expression, synthesizes all mRNA in eukaryotes. The intricate regulation of pol II transcription underlies cell growth and differentiation. Pol II is both a machine, capable of DNA unwinding, RNA polymerization, and proofreading, and also a platform for the assembly of even larger initiation and elongation complexes. The recent structure determination of ten-subunit, 0.5 mDal, Saccharomyces cerevisiae (pol II) at 2.8 ¿ resolution, and of a transcribing pol II complex at 3.3 ¿ resolution, opens the way to many lines of investigation. Structures of cocrystals of pol II with interacting molecules can be solved, the full power of site-directed mutagenesis can be brought to bear on understanding the transcription mechanism, and so forth. Structure determination of the pol II transcription machinery is essential for understanding the mechanism and regulation of transcription.

该副本是使用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。主题项目和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这是调查员的机构。 RNA聚合酶II（POL II），基因表达的中心酶，合成真核生物中的所有mRNA。 Pol II转录的复杂调节是细胞生长和分化的基础。 POL II既是一台机器，能够放松DNA，RNA聚合和校对，也是组装更大的启动和伸长络合物的平台。最新的结构测定在2.8`以2.8»分辨率和以3.3»分辨率转录的Pol II复合物（分辨率为3.3）的十亚基，酿酒酵母（POL II）的结构确定为许多投资打开了道路。可以解决Pol II与相互作用分子的共晶结构，可以使位于定向的诱变的全部力量在理解转录机制之上，等等。 Pol II转录机械的结构确定对于理解转录的机制和调节至关重要。