STRUCTURAL STUDIES OF THE EUKARYOTIC TRANSCRIPTION MACHINERY

真核转录机器的结构研究

• 批准号: 8170090
• 负责人: David A. Bushnell
• 金额: $ 1.76万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170090
• 关键词: Complex; Computer Retrieval of Information on Scientific Projects Database; DNA Polymerase II; Development; Elongation Factor; Enzymes; Eukaryota; Funding; Gene Expression; Genetic Transcription; Grant; Institution; Mediator of activation protein; Messenger RNA; Mutation Analysis; Pathway interactions; Process; Proteins; RNA Polymerase II; Regulation; Research; Research Personnel; Resources; Source; Transcriptional Regulation; United States National Institutes of Health; polypeptide; tumorigenesis

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Transcription is the first step and the key control point in the pathway of gene expression. Transcriptional regulation underlies development, oncogenesis, and other fundamental processes. In eukaryotes the enzyme RNA Polymerase II (pol) is responsible for transcription of messenger RNA making pol?s regulation central to gene expression. During the period of this proposal we will continue our structural studies of the pol transcription machinery. These studies are currently proceeding along three avenues: first, detailed analysis of the elongating pol II using mutation analysis; second, co-crystals of pol II with initiation and elongation factors; third, structural studies of the mediator complex, a 22 polypeptide complex required for pol to respond to regulating proteins.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 转录是基因表达途径中的第一步和关键控制点。转录调节是发展，​​肿瘤发生和其他基本过程的基础。在真核生物中，酶RNA聚合酶II（POL）负责使Messenger RNA的转录使POL的调节与基因表达中心。在此提案期间，我们将继续对POL转录机械进行结构研究。这些研究目前正在沿着三种途径进行：首先使用突变分析对延长的Pol II进行详细分析；其次，Pol II的共结晶与起始和伸长因子；第三，介体复合物的结构研究，这是对调节蛋白质反应的22个多肽复合物。