REDOX IMAGING OF HUMAN LYMPHOMA

人类淋巴瘤的氧化还原成像

• 批准号: 8362023
• 负责人: LIN LI
• 金额: $ 0.77万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362023
• 关键词: Apoptosis; Cell Culture Techniques; Cell physiology; Flavoproteins; Fluorescence; Funding; Grant; Human; Image; Lymphoma; Magnetic Resonance; Measures; Metabolism; Mitochondria; Mus; NADH; National Center for Research Resources; Oxidation-Reduction; Principal Investigator; Research; Research Infrastructure; Resources; Role; Scanning; Signal Transduction; Source; Tissues; United States National Institutes of Health; Xenograft procedure; base; cancer therapy; chemotherapy; cost; in vivo; malignant breast neoplasm; melanoma; novel strategies; optical imaging; response; tumor progression

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Redox scanning can measure the in vivo mitochondrial redox states of tissues on the basis of the fluorescence signals of NADH and Fp (oxidized flavoproteins), and Fp redox ratios (Fp/(NADH+Fp)) was shown by previous studies to predict the aggressiveness of human melanoma and breast cancer in mouse xenografts. Redox ratio is also sensitive to cellular processes including apoptosis, differentiation and metabolism. In this project we employ redox scanning to study human lymphoma cell culture and mouse xenografts with and without chemotherapy. We hope to understand more about the role of mitochondrial redox state in tumor progression and response to treatment. We may also accumulate evidence for targeting mitochondrial redox state as a novel approach of cancer therapy.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 氧化还原扫描可以根据NADH和FP的荧光信号（氧化的黄脂蛋白）和FP氧化还原比（FP/（NADH+FP））的荧光信号测量组织的体内线粒体氧化还原状态，以预测人类黑色素瘤和乳腺癌在小鼠Xenograpts中的侵略性。氧化还原比也对包括凋亡，分化和代谢在内的细胞过程敏感。在这个项目中，我们采用氧化还原扫描来研究有或没有化学疗法的人类淋巴瘤细胞培养和小鼠异种移植物。我们希望更多地了解线粒体氧化还原状态在肿瘤进展和对治疗反应中的作用。我们还可能会积累靶向线粒体氧化还原状态作为癌症治疗方法的证据。